AmpR promoter AmpR promoter(104-208) AmpR ... AmpR promoter(104-208) AmpR ... AmpR promoter(104-208) AmpR AmpR(209-1069) AmpR AmpR(209-1069) ori ori(1240-1828) ori ori(1240-1828) rop rop(2258-2449) rop rop(2258-2449) lacI lacI(3761-4843) lacI lacI(3761-4843) lacI promoter lacI promoter(4844-4921) lacI ... lacI promoter(4844-4921) ...lacI lacI promoter(4844-4921) T7 promoter T7 promoter(5230-5248) T7 pr... T7 promoter(5230-5248) ...T7 pr T7 promoter(5230-5248) lac operator lac operator(5249-5273) lac o... lac operator(5249-5273) ...lac o lac operator(5249-5273) 10xHis 10xHis(5323-5352) 10xHi... 10xHis(5323-5352) ...10xHi 10xHis(5323-5352) enterokinase site enterokinase site(5368-5382) enter... enterokinase site(5368-5382) ...enter enterokinase site(5368-5382) T7 terminator T7 terminator(5458-5505) T7 te... T7 terminator(5458-5505) ...T7 te T7 terminator(5458-5505) tet promoter tet promoter(5680-5708) tet p... tet promoter(5680-5708) ...tet p tet promoter(5680-5708) lac operator lac operator(5255-5271) lac o... lac operator(5255-5271) ...lac o lac operator(5255-5271) T7 promoter T7 promoter(5232-5250) T7 pr... T7 promoter(5232-5250) ...T7 pr T7 promoter(5232-5250) lac operator lac operator(5251-5275) lac o... lac operator(5251-5275) ...lac o lac operator(5251-5275) tet promoter tet promoter(5682-5710) tet p... tet promoter(5682-5710) ...tet p tet promoter(5682-5710) T7 terminator T7 terminator(5460-5507) T7 te... T7 terminator(5460-5507) ...T7 te T7 terminator(5460-5507) lacI promoter lacI promoter(4846-4923) lacI ... lacI promoter(4846-4923) ...lacI lacI promoter(4846-4923) AmpR promoter AmpR promoter(106-210) AmpR ... AmpR promoter(106-210) AmpR ... AmpR promoter(106-210) rop rop(2260-2451) rop rop(2260-2451) ori ori(1242-1830) ori ori(1242-1830) AmpR AmpR(211-1071) AmpR AmpR(211-1071)