f1 ori f1 ori(12-467) f1 or... f1 ori(12-467) AmpR promoter AmpR promoter(494-598) AmpR ... AmpR promoter(494-598) AmpR ... AmpR promoter(494-598) AmpR AmpR(599-1459) AmpR AmpR(599-1459) ori ori(1630-2218) ori ori(1630-2218) rop rop(2648-2839) rop rop(2648-2839) lacI lacI(3648-4730) lacI lacI(3648-4730) lacI promoter lacI promoter(4731-4808) lacI ... lacI promoter(4731-4808) ...lacI lacI promoter(4731-4808) T7 promoter T7 promoter(5117-5135) T7 pr... T7 promoter(5117-5135) ...T7 pr T7 promoter(5117-5135) lac operator lac operator(5136-5160) lac o... lac operator(5136-5160) ...lac o lac operator(5136-5160) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(5205-5237) T7 ta... T7 tag (gene 10 leader)(5205-5237) ...T7 ta T7 tag (gene 10 leader)(5205-5237) 6xHis 6xHis(5287-5304) 6xHis 6xHis(5287-5304) 6xHis 6xHis(5287-5304) T7 terminator T7 terminator(5371-5418) T7 te... T7 terminator(5371-5418) ...T7 te T7 terminator(5371-5418) lac operator lac operator(5142-5158) lac o... lac operator(5142-5158) ...lac o lac operator(5142-5158) T7 promoter T7 promoter(5119-5137) T7 pr... T7 promoter(5119-5137) ...T7 pr T7 promoter(5119-5137) lac operator lac operator(5138-5162) lac o... lac operator(5138-5162) ...lac o lac operator(5138-5162) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(5207-5239) T7 ta... T7 tag (gene 10 leader)(5207-5239) ...T7 ta T7 tag (gene 10 leader)(5207-5239) T7 terminator T7 terminator(5373-5420) T7 te... T7 terminator(5373-5420) ...T7 te T7 terminator(5373-5420) lacI promoter lacI promoter(4733-4810) lacI ... lacI promoter(4733-4810) ...lacI lacI promoter(4733-4810) AmpR promoter AmpR promoter(496-600) AmpR ... AmpR promoter(496-600) AmpR ... AmpR promoter(496-600) rop rop(2650-2841) rop rop(2650-2841) f1 ori f1 ori(14-469) f1 or... f1 ori(14-469) ori ori(1632-2220) ori ori(1632-2220)