MCS MCS(5-61) MCS MCS(5-61) MCS MCS(5-61) SP6 promoter SP6 promoter(68-86) SP6 p... SP6 promoter(68-86) SP6 p... SP6 promoter(68-86) M13 rev M13 rev(104-120) M13 r... M13 rev(104-120) M13 r... M13 rev(104-120) lac operator lac operator(128-144) lac o... lac operator(128-144) lac o... lac operator(128-144) lac promoter lac promoter(152-182) lac p... lac promoter(152-182) lac p... lac promoter(152-182) ori ori(506-1094) ori ori(506-1094) T7 promoter T7 promoter(3181-2) T7 pr... T7 promoter(3181-2) ...T7 pr T7 promoter(3181-2) AmpR AmpR(1265-2125) AmpR AmpR(1265-2125) AmpR promoter AmpR promoter(2126-2230) AmpR ... AmpR promoter(2126-2230) ...AmpR AmpR promoter(2126-2230) f1 ori f1 ori(2562-3017) f1 or... f1 ori(2562-3017) M13 fwd M13 fwd(3158-3174) M13 f... M13 fwd(3158-3174) ...M13 f M13 fwd(3158-3174) M13 rev M13 rev(106-122) M13 r... M13 rev(106-122) M13 r... M13 rev(106-122) M13 fwd M13 fwd(3160-3176) M13 f... M13 fwd(3160-3176) ...M13 f M13 fwd(3160-3176) lac operator lac operator(130-146) lac o... lac operator(130-146) lac o... lac operator(130-146) SP6 promoter SP6 promoter(70-88) SP6 p... SP6 promoter(70-88) SP6 p... SP6 promoter(70-88) CAP binding site CAP binding site(199-220) CAP b... CAP binding site(199-220) CAP b... CAP binding site(199-220) lac promoter lac promoter(154-184) lac p... lac promoter(154-184) ...lac p lac promoter(154-184) MCS MCS(7-63) MCS MCS(7-63) MCS MCS(7-63) MCS MCS(7-63) MCS MCS(7-63) MCS MCS(7-63) AmpR promoter AmpR promoter(2128-2232) AmpR ... AmpR promoter(2128-2232) ...AmpR AmpR promoter(2128-2232)