f1 ori f1 ori(12-467) f1 or... f1 ori(12-467) KanR KanR(560-1375) KanR KanR(560-1375) ori ori(1497-2085) ori ori(1497-2085) rop rop(2515-2706) rop rop(2515-2706) lacI lacI(3515-4597) lacI lacI(3515-4597) lacI promoter lacI promoter(4598-4675) lacI ... lacI promoter(4598-4675) ...lacI lacI promoter(4598-4675) T7 promoter T7 promoter(4984-5002) T7 pr... T7 promoter(4984-5002) ...T7 pr T7 promoter(4984-5002) lac operator lac operator(5003-5027) lac o... lac operator(5003-5027) ...lac o lac operator(5003-5027) 6xHis 6xHis(5083-5100) 6xHis 6xHis(5083-5100) 6xHis 6xHis(5083-5100) thrombin site thrombin site(5110-5127) throm... thrombin site(5110-5127) ...throm thrombin site(5110-5127) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(5131-5163) T7 ta... T7 tag (gene 10 leader)(5131-5163) ...T7 ta T7 tag (gene 10 leader)(5131-5163) 6xHis 6xHis(5213-5230) 6xHis 6xHis(5213-5230) 6xHis 6xHis(5213-5230) T7 terminator T7 terminator(5297-5344) T7 te... T7 terminator(5297-5344) ...T7 te T7 terminator(5297-5344) lac operator lac operator(5009-5025) lac o... lac operator(5009-5025) ...lac o lac operator(5009-5025) thrombin site thrombin site(5112-5129) throm... thrombin site(5112-5129) ...throm thrombin site(5112-5129) T7 promoter T7 promoter(4986-5004) T7 pr... T7 promoter(4986-5004) ...T7 pr T7 promoter(4986-5004) lac operator lac operator(5005-5029) lac o... lac operator(5005-5029) ...lac o lac operator(5005-5029) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(5133-5165) T7 ta... T7 tag (gene 10 leader)(5133-5165) ...T7 ta T7 tag (gene 10 leader)(5133-5165) T7 terminator T7 terminator(5299-5346) T7 te... T7 terminator(5299-5346) ...T7 te T7 terminator(5299-5346) lacI promoter lacI promoter(4600-4677) lacI ... lacI promoter(4600-4677) ...lacI lacI promoter(4600-4677) rop rop(2517-2708) rop rop(2517-2708) f1 ori f1 ori(14-469) f1 or... f1 ori(14-469) ori ori(1499-2087) ori ori(1499-2087)