f1 ori f1 ori(12-467) f1 or... f1 ori(12-467) KanR KanR(560-1375) KanR KanR(560-1375) ori ori(1497-2085) ori ori(1497-2085) rop rop(2515-2706) rop rop(2515-2706) lacI lacI(3515-4597) lacI lacI(3515-4597) lacI promoter lacI promoter(4598-4675) lacI ... lacI promoter(4598-4675) ...lacI lacI promoter(4598-4675) T7 promoter T7 promoter(4988-5006) T7 pr... T7 promoter(4988-5006) ...T7 pr T7 promoter(4988-5006) lac operator lac operator(5007-5031) lac o... lac operator(5007-5031) ...lac o lac operator(5007-5031) S-Tag S-Tag(5079-5123) S-Tag S-Tag(5079-5123) S-Tag S-Tag(5079-5123) thrombin site thrombin site(5139-5156) throm... thrombin site(5139-5156) ...throm thrombin site(5139-5156) 6xHis 6xHis(5215-5232) 6xHis 6xHis(5215-5232) 6xHis 6xHis(5215-5232) T7 terminator T7 terminator(5299-5346) T7 te... T7 terminator(5299-5346) ...T7 te T7 terminator(5299-5346) lac operator lac operator(5013-5029) lac o... lac operator(5013-5029) ...lac o lac operator(5013-5029) T7 promoter T7 promoter(4990-5008) T7 pr... T7 promoter(4990-5008) ...T7 pr T7 promoter(4990-5008) lac operator lac operator(5009-5033) lac o... lac operator(5009-5033) ...lac o lac operator(5009-5033) S-Tag S-Tag(5081-5125) S-Tag S-Tag(5081-5125) S-Tag S-Tag(5081-5125) T7 terminator T7 terminator(5301-5348) T7 te... T7 terminator(5301-5348) ...T7 te T7 terminator(5301-5348) lacI promoter lacI promoter(4600-4677) lacI ... lacI promoter(4600-4677) ...lacI lacI promoter(4600-4677) rop rop(2517-2708) rop rop(2517-2708) f1 ori f1 ori(14-469) f1 or... f1 ori(14-469) ori ori(1499-2087) ori ori(1499-2087)