f1 ori f1 ori(12-467) f1 or... f1 ori(12-467) KanR KanR(560-1375) KanR KanR(560-1375) ori ori(1497-2085) ori ori(1497-2085) rop rop(2515-2706) rop rop(2515-2706) lacI lacI(3515-4597) lacI lacI(3515-4597) lacI promoter lacI promoter(4598-4675) lacI ... lacI promoter(4598-4675) ...lacI lacI promoter(4598-4675) T7 promoter T7 promoter(4988-5006) T7 pr... T7 promoter(4988-5006) ...T7 pr T7 promoter(4988-5006) lac operator lac operator(5007-5031) lac o... lac operator(5007-5031) ...lac o lac operator(5007-5031) 6xHis 6xHis(5079-5096) 6xHis 6xHis(5079-5096) 6xHis 6xHis(5079-5096) thrombin site thrombin site(5106-5123) throm... thrombin site(5106-5123) ...throm thrombin site(5106-5123) S-Tag S-Tag(5130-5174) S-Tag S-Tag(5130-5174) S-Tag S-Tag(5130-5174) enterokinase site enterokinase site(5190-5204) enter... enterokinase site(5190-5204) ...enter enterokinase site(5190-5204) 6xHis 6xHis(5265-5282) 6xHis 6xHis(5265-5282) 6xHis 6xHis(5265-5282) T7 terminator T7 terminator(5349-5396) T7 te... T7 terminator(5349-5396) ...T7 te T7 terminator(5349-5396) lac operator lac operator(5013-5029) lac o... lac operator(5013-5029) ...lac o lac operator(5013-5029) T7 promoter T7 promoter(4990-5008) T7 pr... T7 promoter(4990-5008) ...T7 pr T7 promoter(4990-5008) lac operator lac operator(5009-5033) lac o... lac operator(5009-5033) ...lac o lac operator(5009-5033) S-Tag S-Tag(5132-5176) S-Tag S-Tag(5132-5176) S-Tag S-Tag(5132-5176) T7 terminator T7 terminator(5351-5398) T7 te... T7 terminator(5351-5398) ...T7 te T7 terminator(5351-5398) lacI promoter lacI promoter(4600-4677) lacI ... lacI promoter(4600-4677) ...lacI lacI promoter(4600-4677) rop rop(2517-2708) rop rop(2517-2708) f1 ori f1 ori(14-469) f1 or... f1 ori(14-469) ori ori(1499-2087) ori ori(1499-2087)