f1 ori f1 ori(12-467) f1 or... f1 ori(12-467) KanR KanR(560-1375) KanR KanR(560-1375) ori ori(1497-2085) ori ori(1497-2085) rop rop(2515-2706) rop rop(2515-2706) lacI lacI(3515-4597) lacI lacI(3515-4597) lacI promoter lacI promoter(4598-4675) lacI ... lacI promoter(4598-4675) ...lacI lacI promoter(4598-4675) T7 promoter T7 promoter(4988-5006) T7 pr... T7 promoter(4988-5006) ...T7 pr T7 promoter(4988-5006) lac operator lac operator(5007-5031) lac o... lac operator(5007-5031) ...lac o lac operator(5007-5031) DsbC DsbC(5076-5783) DsbC DsbC(5076-5783) 6xHis 6xHis(5805-5822) 6xHis 6xHis(5805-5822) 6xHis 6xHis(5805-5822) thrombin site thrombin site(5832-5849) throm... thrombin site(5832-5849) ...throm thrombin site(5832-5849) S-Tag S-Tag(5865-5909) S-Tag S-Tag(5865-5909) S-Tag S-Tag(5865-5909) enterokinase site enterokinase site(5925-5939) enter... enterokinase site(5925-5939) ...enter enterokinase site(5925-5939) 8xHis 8xHis(6018-6041) 8xHis 8xHis(6018-6041) 8xHis 8xHis(6018-6041) T7 terminator T7 terminator(6118-6165) T7 te... T7 terminator(6118-6165) ...T7 te T7 terminator(6118-6165) lac operator lac operator(5013-5029) lac o... lac operator(5013-5029) ...lac o lac operator(5013-5029) T7 promoter T7 promoter(4990-5008) T7 pr... T7 promoter(4990-5008) ...T7 pr T7 promoter(4990-5008) lac operator lac operator(5009-5033) lac o... lac operator(5009-5033) ...lac o lac operator(5009-5033) S-Tag S-Tag(5867-5911) S-Tag S-Tag(5867-5911) S-Tag S-Tag(5867-5911) T7 terminator T7 terminator(6120-6167) T7 te... T7 terminator(6120-6167) ...T7 te T7 terminator(6120-6167) lacI promoter lacI promoter(4600-4677) lacI ... lacI promoter(4600-4677) ...lacI lacI promoter(4600-4677) rop rop(2517-2708) rop rop(2517-2708) f1 ori f1 ori(14-469) f1 or... f1 ori(14-469) ori ori(1499-2087) ori ori(1499-2087) DsbC DsbC(5078-5785) DsbC DsbC(5078-5785)