f1 ori f1 ori(12-467) f1 or... f1 ori(12-467) KanR KanR(560-1375) KanR KanR(560-1375) ori ori(1497-2085) ori ori(1497-2085) rop rop(2515-2706) rop rop(2515-2706) lacI lacI(3278-4360) lacI lacI(3278-4360) lacI promoter lacI promoter(4361-4438) lacI ... lacI promoter(4361-4438) ...lacI lacI promoter(4361-4438) T7 promoter T7 promoter(4751-4769) T7 pr... T7 promoter(4751-4769) ...T7 pr T7 promoter(4751-4769) lac operator lac operator(4770-4794) lac o... lac operator(4770-4794) ...lac o lac operator(4770-4794) GST GST(4839-5492) GST GST(4839-5492) 6xHis 6xHis(5520-5537) 6xHis 6xHis(5520-5537) 6xHis 6xHis(5520-5537) thrombin site thrombin site(5547-5564) throm... thrombin site(5547-5564) ...throm thrombin site(5547-5564) S-Tag S-Tag(5580-5624) S-Tag S-Tag(5580-5624) S-Tag S-Tag(5580-5624) enterokinase site enterokinase site(5655-5669) enter... enterokinase site(5655-5669) ...enter enterokinase site(5655-5669) 8xHis 8xHis(5761-5784) 8xHis 8xHis(5761-5784) 8xHis 8xHis(5761-5784) T7 terminator T7 terminator(5861-5908) T7 te... T7 terminator(5861-5908) ...T7 te T7 terminator(5861-5908) lac operator lac operator(4776-4792) lac o... lac operator(4776-4792) ...lac o lac operator(4776-4792) T7 promoter T7 promoter(4753-4771) T7 pr... T7 promoter(4753-4771) ...T7 pr T7 promoter(4753-4771) lac operator lac operator(4772-4796) lac o... lac operator(4772-4796) ...lac o lac operator(4772-4796) S-Tag S-Tag(5582-5626) S-Tag S-Tag(5582-5626) S-Tag S-Tag(5582-5626) T7 terminator T7 terminator(5863-5910) T7 te... T7 terminator(5863-5910) ...T7 te T7 terminator(5863-5910) lacI promoter lacI promoter(4363-4440) lacI ... lacI promoter(4363-4440) ...lacI lacI promoter(4363-4440) rop rop(2517-2708) rop rop(2517-2708) f1 ori f1 ori(14-469) f1 or... f1 ori(14-469) ori ori(1499-2087) ori ori(1499-2087)