f1 ori f1 ori(12-467) f1 or... f1 ori(12-467) AmpR AmpR(582-1442) AmpR AmpR(582-1442) AmpR promoter AmpR promoter(1443-1535) AmpR ... AmpR promoter(1443-1535) AmpR ... AmpR promoter(1443-1535) ori ori(1616-2204) ori ori(1616-2204) rop rop(2634-2825) rop rop(2634-2825) lacI lacI(3397-4479) lacI lacI(3397-4479) lacI promoter lacI promoter(4480-4557) lacI ... lacI promoter(4480-4557) ...lacI lacI promoter(4480-4557) T7 promoter T7 promoter(4870-4888) T7 pr... T7 promoter(4870-4888) ...T7 pr T7 promoter(4870-4888) lac operator lac operator(4889-4913) lac o... lac operator(4889-4913) ...lac o lac operator(4889-4913) 6xHis 6xHis(4963-4980) 6xHis 6xHis(4963-4980) 6xHis 6xHis(4963-4980) enterokinase site enterokinase site(4999-5013) enter... enterokinase site(4999-5013) ...enter enterokinase site(4999-5013) S-Tag S-Tag(5092-5136) S-Tag S-Tag(5092-5136) S-Tag S-Tag(5092-5136) T7 terminator T7 terminator(5188-5235) T7 te... T7 terminator(5188-5235) ...T7 te T7 terminator(5188-5235) lac operator lac operator(4895-4911) lac o... lac operator(4895-4911) ...lac o lac operator(4895-4911) T7 promoter T7 promoter(4872-4890) T7 pr... T7 promoter(4872-4890) ...T7 pr T7 promoter(4872-4890) lac operator lac operator(4891-4915) lac o... lac operator(4891-4915) ...lac o lac operator(4891-4915) T7 terminator T7 terminator(5190-5237) T7 te... T7 terminator(5190-5237) ...T7 te T7 terminator(5190-5237) lacI promoter lacI promoter(4482-4559) lacI ... lacI promoter(4482-4559) ...lacI lacI promoter(4482-4559) rop rop(2636-2827) rop rop(2636-2827) f1 ori f1 ori(14-469) f1 or... f1 ori(14-469) ori ori(1618-2206) ori ori(1618-2206)