lac operator lac operator(3-27) lac o... lac operator(3-27) lac o... lac operator(3-27) 6xHis 6xHis(83-100) 6xHis 6xHis(83-100) 6xHis 6xHis(83-100) T7 promoter T7 promoter(214-232) T7 pr... T7 promoter(214-232) T7 pr... T7 promoter(214-232) lac operator lac operator(233-257) lac o... lac operator(233-257) lac o... lac operator(233-257) S-Tag S-Tag(366-410) S-Tag S-Tag(366-410) S-Tag S-Tag(366-410) T7 terminator T7 terminator(462-509) T7 te... T7 terminator(462-509) T7 te... T7 terminator(462-509) f1 ori f1 ori(546-1001) f1 or... f1 ori(546-1001) AmpR AmpR(1116-1976) AmpR AmpR(1116-1976) AmpR promoter AmpR promoter(1977-2069) AmpR ... AmpR promoter(1977-2069) AmpR ... AmpR promoter(1977-2069) ori ori(2150-2738) ori ori(2150-2738) T7 promoter T7 promoter(5404-2) T7 pr... T7 promoter(5404-2) ...T7 pr T7 promoter(5404-2) rop rop(3168-3359) rop rop(3168-3359) lacI lacI(3931-5013) lacI lacI(3931-5013) lacI promoter lacI promoter(5014-5091) lacI ... lacI promoter(5014-5091) ...lacI lacI promoter(5014-5091) lac operator lac operator(9-25) lac o... lac operator(9-25) lac o... lac operator(9-25) lac operator lac operator(239-255) lac o... lac operator(239-255) lac o... lac operator(239-255) T7 promoter T7 promoter(216-234) T7 pr... T7 promoter(216-234) T7 pr... T7 promoter(216-234) lac operator lac operator(5-29) lac o... lac operator(5-29) lac o... lac operator(5-29) lac operator lac operator(235-259) lac o... lac operator(235-259) lac o... lac operator(235-259) T7 terminator T7 terminator(464-511) T7 te... T7 terminator(464-511) T7 te... T7 terminator(464-511) lacI promoter lacI promoter(5016-5093) lacI ... lacI promoter(5016-5093) ...lacI lacI promoter(5016-5093) rop rop(3170-3361) rop rop(3170-3361) f1 ori f1 ori(548-1003) f1 or... f1 ori(548-1003) ori ori(2152-2740) ori ori(2152-2740)