f1 ori f1 ori(12-467) f1 or... f1 ori(12-467) AmpR AmpR(582-1442) AmpR AmpR(582-1442) AmpR promoter AmpR promoter(1443-1535) AmpR ... AmpR promoter(1443-1535) AmpR ... AmpR promoter(1443-1535) ori ori(1616-2204) ori ori(1616-2204) rop rop(2634-2825) rop rop(2634-2825) lacI lacI(3397-4479) lacI lacI(3397-4479) lacI promoter lacI promoter(4480-4557) lacI ... lacI promoter(4480-4557) ...lacI lacI promoter(4480-4557) T7 promoter T7 promoter(4870-4888) T7 pr... T7 promoter(4870-4888) ...T7 pr T7 promoter(4870-4888) lac operator lac operator(4889-4913) lac o... lac operator(4889-4913) ...lac o lac operator(4889-4913) 6xHis 6xHis(4970-4987) 6xHis 6xHis(4970-4987) 6xHis 6xHis(4970-4987) NusA NusA(4994-6478) NusA NusA(4994-6478) 6xHis 6xHis(6494-6511) 6xHis 6xHis(6494-6511) 6xHis 6xHis(6494-6511) S-Tag S-Tag(6521-6565) S-Tag S-Tag(6521-6565) S-Tag S-Tag(6521-6565) thrombin site thrombin site(6581-6598) throm... thrombin site(6581-6598) ...throm thrombin site(6581-6598) enterokinase site enterokinase site(6617-6631) enter... enterokinase site(6617-6631) ...enter enterokinase site(6617-6631) HSV tag HSV tag(6743-6775) HSV t... HSV tag(6743-6775) ...HSV t HSV tag(6743-6775) 6xHis 6xHis(6782-6799) 6xHis 6xHis(6782-6799) 6xHis 6xHis(6782-6799) rrnB T1 terminator rrnB T1 terminator(6965-7051) rrnB ... rrnB T1 terminator(6965-7051) ...rrnB rrnB T1 terminator(6965-7051) T7 terminator T7 terminator(7238-7285) T7 te... T7 terminator(7238-7285) ...T7 te T7 terminator(7238-7285) lac operator lac operator(4895-4911) lac o... lac operator(4895-4911) ...lac o lac operator(4895-4911) T7 promoter T7 promoter(4872-4890) T7 pr... T7 promoter(4872-4890) ...T7 pr T7 promoter(4872-4890) lac operator lac operator(4891-4915) lac o... lac operator(4891-4915) ...lac o lac operator(4891-4915) rrnB T2 terminator rrnB T2 terminator(7145-7172) rrnB ... rrnB T2 terminator(7145-7172) ...rrnB rrnB T2 terminator(7145-7172) T7 terminator T7 terminator(7240-7287) T7 te... T7 terminator(7240-7287) ...T7 te T7 terminator(7240-7287) lacI promoter lacI promoter(4482-4559) lacI ... lacI promoter(4482-4559) ...lacI lacI promoter(4482-4559) rrnB T1 terminator rrnB T1 terminator(6967-7053) rrnB ... rrnB T1 terminator(6967-7053) ...rrnB rrnB T1 terminator(6967-7053) rop rop(2636-2827) rop rop(2636-2827) f1 ori f1 ori(14-469) f1 or... f1 ori(14-469) ori ori(1618-2206) ori ori(1618-2206) NusA NusA(4996-6480) NusA NusA(4996-6480)