trc promoter trc promoter(193-222) trc p... trc promoter(193-222) trc p... trc promoter(193-222) lac operator lac operator(230-246) lac o... lac operator(230-246) lac o... lac operator(230-246) MCS MCS(270-326) MCS MCS(270-326) MCS MCS(270-326) rrnB T1 terminator rrnB T1 terminator(529-615) rrnB ... rrnB T1 terminator(529-615) rrnB ... rrnB T1 terminator(529-615) AmpR promoter AmpR promoter(754-845) AmpR ... AmpR promoter(754-845) AmpR ... AmpR promoter(754-845) AmpR AmpR(846-1706) AmpR AmpR(846-1706) ori ori(1877-2465) ori ori(1877-2465) lacIq promoter lacIq promoter(2977-3054) lacIq... lacIq promoter(2977-3054) ...lacIq lacIq promoter(2977-3054) lacI lacI(3055-4137) lacI lacI(3055-4137) lac operator lac operator(232-248) lac o... lac operator(232-248) lac o... lac operator(232-248) rrnB T2 terminator rrnB T2 terminator(709-736) rrnB ... rrnB T2 terminator(709-736) rrnB ... rrnB T2 terminator(709-736) trc promoter trc promoter(195-224) trc p... trc promoter(195-224) trc p... trc promoter(195-224) MCS MCS(272-328) MCS MCS(272-328) MCS MCS(272-328) MCS MCS(272-328) MCS MCS(272-328) MCS MCS(272-328) lacIq promoter lacIq promoter(2979-3056) lacIq... lacIq promoter(2979-3056) ...lacIq lacIq promoter(2979-3056) rrnB T1 terminator rrnB T1 terminator(531-617) rrnB ... rrnB T1 terminator(531-617) rrnB ... rrnB T1 terminator(531-617) bom bom(2653-2793) bom bom(2653-2793) ori ori(1879-2467) ori ori(1879-2467)