lacIq promoter lacIq promoter(3-80) lacIq... lacIq promoter(3-80) lacIq... lacIq promoter(3-80) lacI lacI(81-1163) lacI lacI(81-1163) tac promoter tac promoter(1406-1434) tac p... tac promoter(1406-1434) tac p... tac promoter(1406-1434) lac operator lac operator(1442-1458) lac o... lac operator(1442-1458) lac o... lac operator(1442-1458) MBP MBP(1528-2628) MBP MBP(1528-2628) Factor Xa site Factor Xa site(2677-2688) Facto... Factor Xa site(2677-2688) Facto... Factor Xa site(2677-2688) M13 fwd M13 fwd(2736-2752) M13 f... M13 fwd(2736-2752) M13 f... M13 fwd(2736-2752) rrnB T1 terminator rrnB T1 terminator(3101-3187) rrnB ... rrnB T1 terminator(3101-3187) rrnB ... rrnB T1 terminator(3101-3187) AmpR promoter AmpR promoter(3326-3417) AmpR ... AmpR promoter(3326-3417) ...AmpR AmpR promoter(3326-3417) AmpR AmpR(3418-4278) AmpR AmpR(3418-4278) f1 ori f1 ori(4378-4833) f1 or... f1 ori(4378-4833) ori ori(4944-5532) ori ori(4944-5532) rop rop(5962-6153) rop rop(5962-6153) M13 fwd M13 fwd(2738-2754) M13 f... M13 fwd(2738-2754) M13 f... M13 fwd(2738-2754) lac operator lac operator(1444-1460) lac o... lac operator(1444-1460) lac o... lac operator(1444-1460) rrnB T2 terminator rrnB T2 terminator(3281-3308) rrnB ... rrnB T2 terminator(3281-3308) rrnB ... rrnB T2 terminator(3281-3308) tac promoter tac promoter(1408-1436) tac p... tac promoter(1408-1436) tac p... tac promoter(1408-1436) lacIq promoter lacIq promoter(5-82) lacIq... lacIq promoter(5-82) lacIq... lacIq promoter(5-82) rrnB T1 terminator rrnB T1 terminator(3103-3189) rrnB ... rrnB T1 terminator(3103-3189) rrnB ... rrnB T1 terminator(3103-3189) ori ori(4946-5534) ori ori(4946-5534)