M13 fwd(379-395) M13 fwd M13 fwd(379-395) M13 fwd M13 fwd(379-395) M13 fwd M13 fwd(379-395) MCS(396-476) MCS MCS(396-476) MCS MCS(396-476) M13 rev(489-505) M13 rev M13 rev(489-505) M13 rev M13 rev(489-505) M13 rev M13 rev(489-505) lac operator(513-529) lac operator lac operator(513-529) lac operator lac operator(513-529) lac operator lac operator(513-529) lac promoter(537-567) lac promoter lac promoter(537-567) lac promoter lac promoter(537-567) lac promoter lac promoter(537-567) ori(891-1479) ori ori(891-1479) ori ori(891-1479) AmpR(1650-2510) AmpR AmpR(1650-2510) AmpR AmpR(1650-2510) AmpR promoter(2511-2615) AmpR promoter AmpR promoter(2511-2615) AmpR promoter AmpR promoter(2511-2615) AmpR promoter AmpR promoter(2511-2615) M13 rev(491-507) M13 rev M13 rev(491-507) M13 rev M13 rev(491-507) M13 rev M13 rev(491-507) M13 fwd(381-397) M13 fwd M13 fwd(381-397) M13 fwd M13 fwd(381-397) M13 fwd M13 fwd(381-397) lac operator(515-531) lac operator lac operator(515-531) lac operator lac operator(515-531) lac operator lac operator(515-531) CAP binding site(584-605) CAP binding site CAP binding site(584-605) CAP binding site CAP binding site(584-605) CAP binding site CAP binding site(584-605) lac promoter(539-569) lac promoter lac promoter(539-569) lac promoter lac promoter(539-569) lac promoter lac promoter(539-569) AmpR promoter(2513-2617) AmpR promoter AmpR promoter(2513-2617) AmpR promoter AmpR promoter(2513-2617) AmpR promoter AmpR promoter(2513-2617)