AmpR promoter(96-200) AmpR promoter AmpR promoter(96-200) AmpR promoter AmpR promoter(96-200) AmpR promoter AmpR promoter(96-200) AmpR(201-1061) AmpR AmpR(201-1061) AmpR AmpR(201-1061) ori(1232-1820) ori ori(1232-1820) ori ori(1232-1820) CAP binding site(2108-2129) CAP binding site CAP binding site(2108-2129) CAP binding site CAP binding site(2108-2129) CAP binding site CAP binding site(2108-2129) lac promoter(2144-2174) lac promoter lac promoter(2144-2174) lac promoter lac promoter(2144-2174) lac promoter lac promoter(2144-2174) lac operator(2182-2198) lac operator lac operator(2182-2198) lac operator lac operator(2182-2198) lac operator lac operator(2182-2198) M13 rev(2206-2222) M13 rev M13 rev(2206-2222) M13 rev M13 rev(2206-2222) M13 rev M13 rev(2206-2222) attB(2276-2309) attB attB(2276-2309) attB attB(2276-2309) attB attB(2276-2309) dU6-2 promoter(2319-2718) dU6-2 promoter dU6-2 promoter(2319-2718) dU6-2 promoter dU6-2 promoter(2319-2718) dU6-2 promoter dU6-2 promoter(2319-2718) gRNA scaffold(2741-2816) gRNA scaffold gRNA scaffold(2741-2816) gRNA scaffold gRNA scaffold(2741-2816) gRNA scaffold gRNA scaffold(2741-2816) Ac5 promoter(2965-5474) Ac5 promoter Ac5 promoter(2965-5474) Ac5 promoter Ac5 promoter(2965-5474) EGFP(5506-6222) EGFP EGFP(5506-6222) EGFP EGFP(5506-6222) T2A(6226-6279) T2A T2A(6226-6279) T2A T2A(6226-6279) T2A T2A(6226-6279) PuroR(6304-6903) PuroR PuroR(6304-6903) PuroR PuroR(6304-6903) SV40 poly(A) signal(6911-7045) SV40 poly(A) signal SV40 poly(A) signal(6911-7045) SV40 poly(A) signal SV40 poly(A) signal(6911-7045) SV40 poly(A) signal SV40 poly(A) signal(6911-7045) attB(7239-7272) attB attB(7239-7272) attB attB(7239-7272) attB attB(7239-7272) M13 fwd(7288-7304) M13 fwd M13 fwd(7288-7304) M13 fwd M13 fwd(7288-7304) M13 fwd M13 fwd(7288-7304) M13 rev(2208-2224) M13 rev M13 rev(2208-2224) M13 rev M13 rev(2208-2224) M13 rev M13 rev(2208-2224) M13 fwd(7290-7306) M13 fwd M13 fwd(7290-7306) M13 fwd M13 fwd(7290-7306) M13 fwd M13 fwd(7290-7306) lac operator(2184-2200) lac operator lac operator(2184-2200) lac operator lac operator(2184-2200) lac operator lac operator(2184-2200) CAP binding site(2110-2131) CAP binding site CAP binding site(2110-2131) CAP binding site CAP binding site(2110-2131) CAP binding site CAP binding site(2110-2131) lac promoter(2146-2176) lac promoter lac promoter(2146-2176) lac promoter lac promoter(2146-2176) lac promoter lac promoter(2146-2176) gRNA scaffold(2743-2818) gRNA scaffold gRNA scaffold(2743-2818) gRNA scaffold gRNA scaffold(2743-2818) gRNA scaffold gRNA scaffold(2743-2818) AmpR promoter(98-202) AmpR promoter AmpR promoter(98-202) AmpR promoter AmpR promoter(98-202) AmpR promoter AmpR promoter(98-202) SV40 poly(A) signal(6913-7047) SV40 poly(A) signal SV40 poly(A) signal(6913-7047) SV40 poly(A) signal SV40 poly(A) signal(6913-7047) SV40 poly(A) signal SV40 poly(A) signal(6913-7047) PuroR(6306-6905) PuroR PuroR(6306-6905) PuroR PuroR(6306-6905)