U6 promoter(94-342) U6 promoter U6 promoter(94-342) U6 promoter U6 promoter(94-342) U6 promoter U6 promoter(94-342) CMV enhancer(514-817) CMV enhancer CMV enhancer(514-817) CMV enhancer CMV enhancer(514-817) CMV enhancer CMV enhancer(514-817) chimeric intron(1095-2111) chimeric intron chimeric intron(1095-2111) chimeric intron chimeric intron(1095-2111) SV40 NLS(2215-2235) SV40 NLS SV40 NLS(2215-2235) SV40 NLS SV40 NLS(2215-2235) SV40 NLS SV40 NLS(2215-2235) St1Cas9(3436-6795) St1Cas9 St1Cas9(3436-6795) St1Cas9 St1Cas9(3436-6795) SV40 NLS(6808-6828) SV40 NLS SV40 NLS(6808-6828) SV40 NLS SV40 NLS(6808-6828) SV40 NLS SV40 NLS(6808-6828) β-globin poly(A) signal(6982-7037) β-globin poly(A) signal β-globin poly(A) signal(6982-7037) β-globin poly(A) signal β-globin poly(A) signal(6982-7037) β-globin poly(A) signal β-globin poly(A) signal(6982-7037) M13 rev(7398-7414) M13 rev M13 rev(7398-7414) M13 rev M13 rev(7398-7414) M13 rev M13 rev(7398-7414) lac operator(7422-7438) lac operator lac operator(7422-7438) lac operator lac operator(7422-7438) lac operator lac operator(7422-7438) lac promoter(7446-7476) lac promoter lac promoter(7446-7476) lac promoter lac promoter(7446-7476) lac promoter lac promoter(7446-7476) CAP binding site(7491-7512) CAP binding site CAP binding site(7491-7512) CAP binding site CAP binding site(7491-7512) CAP binding site CAP binding site(7491-7512) SV40 promoter(7571-7766) SV40 promoter SV40 promoter(7571-7766) SV40 promoter SV40 promoter(7571-7766) SV40 promoter SV40 promoter(7571-7766) SV40 ori(7617-7752) SV40 ori SV40 ori(7617-7752) SV40 ori SV40 ori(7617-7752) SV40 ori SV40 ori(7617-7752) SV40 poly(A) signal(7772-7906) SV40 poly(A) signal SV40 poly(A) signal(7772-7906) SV40 poly(A) signal SV40 poly(A) signal(7772-7906) SV40 poly(A) signal SV40 poly(A) signal(7772-7906) ori(8145-8733) ori ori(8145-8733) ori ori(8145-8733) AmpR(8904-9764) AmpR AmpR(8904-9764) AmpR AmpR(8904-9764) AmpR promoter(9765-9869) AmpR promoter AmpR promoter(9765-9869) AmpR promoter AmpR promoter(9765-9869) AmpR promoter AmpR promoter(9765-9869) M13 rev(7400-7416) M13 rev M13 rev(7400-7416) M13 rev M13 rev(7400-7416) M13 rev M13 rev(7400-7416) lac operator(7424-7440) lac operator lac operator(7424-7440) lac operator lac operator(7424-7440) lac operator lac operator(7424-7440) CAP binding site(7493-7514) CAP binding site CAP binding site(7493-7514) CAP binding site CAP binding site(7493-7514) CAP binding site CAP binding site(7493-7514) lac promoter(7448-7478) lac promoter lac promoter(7448-7478) lac promoter lac promoter(7448-7478) lac promoter lac promoter(7448-7478) β-globin poly(A) signal(6984-7039) β-globin poly(A) signal β-globin poly(A) signal(6984-7039) β-globin poly(A) signal β-globin poly(A) signal(6984-7039) β-globin poly(A) signal β-globin poly(A) signal(6984-7039) AmpR promoter(9767-9871) AmpR promoter AmpR promoter(9767-9871) AmpR promoter AmpR promoter(9767-9871) AmpR promoter AmpR promoter(9767-9871) SV40 poly(A) signal(7774-7908) SV40 poly(A) signal SV40 poly(A) signal(7774-7908) SV40 poly(A) signal SV40 poly(A) signal(7774-7908) SV40 poly(A) signal SV40 poly(A) signal(7774-7908) SV40 ori(7619-7754) SV40 ori SV40 ori(7619-7754) SV40 ori SV40 ori(7619-7754) SV40 ori SV40 ori(7619-7754) U6 promoter(96-336) U6 promoter U6 promoter(96-336) U6 promoter U6 promoter(96-336) U6 promoter U6 promoter(96-336)