Design Vector
Editing vector（DNA level）-Nematode-Enem02
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M13 fwd(3-19)
M13 fwd
M13 fwd(3-19)
M13 fwd
M13 fwd(3-19)
M13 fwd
M13 fwd(3-19)
gRNA scaffold(64-139)
gRNA scaffold
gRNA scaffold(64-139)
gRNA scaffold
gRNA scaffold(64-139)
gRNA sc...
gRNA scaffold(64-139)
T3 promoter(157-175)
T3 promoter
T3 promoter(157-175)
T3 promoter
T3 promoter(157-175)
T3 promoter
T3 promoter(157-175)
M13 rev(196-212)
M13 rev
M13 rev(196-212)
M13 rev
M13 rev(196-212)
M13 rev
M13 rev(196-212)
ori(379-967)
ori
ori(379-967)
ori
ori(379-967)
ori
ori(379-967)
NeoR/KanR(1141-1935)
NeoR/KanR
NeoR/KanR(1141-1935)
NeoR/KanR
NeoR/KanR(1141-1935)
NeoR/KanR
NeoR/KanR(1141-1935)
AmpR promoter(1936-2040)
AmpR promoter
AmpR promoter(1936-2040)
AmpR promoter
AmpR promoter(1936-2040)
AmpR promoter
AmpR promoter(1936-2040)
M13 rev(198-214)
M13 rev
M13 rev(198-214)
M13 rev
M13 rev(198-214)
M13 rev
M13 rev(198-214)
M13 fwd(5-21)
M13 fwd
M13 fwd(5-21)
M13 fwd
M13 fwd(5-21)
M13 fwd
M13 fwd(5-21)
T3 promoter(159-177)
T3 promoter
T3 promoter(159-177)
T3 promoter
T3 promoter(159-177)
T3 promoter
T3 promoter(159-177)
gRNA scaffold(66-141)
gRNA scaffold
gRNA scaffold(66-141)
gRNA scaffold
gRNA scaffold(66-141)
gRNA scaffold
gRNA scaffold(66-141)
AmpR promoter(1938-2042)
AmpR promoter
AmpR promoter(1938-2042)
AmpR promoter
AmpR promoter(1938-2042)
AmpR promoter
AmpR promoter(1938-2042)
Select from database
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Select from database
Mammalian constitutive expression promoters
Mammalian tissue-specific expression promoters
Mammalian inducible expression promoters
Zebrafish constitutive expression promoters
Zebrafish inducible expression promoters
Plant constitutive expression promoters
Yeast constitutive expression promoters
Yeast inducible expression promoters
Drosophila constitutive expression promoters
Drosophila tissue-specific promoters
Drosophila inducible expression promoters
Drosophila Pol III promoters
Plant Pol III promoters
C. elegans constitutive expression promoters
C. elegans tissue-specific expression promoters
C. elegans inducible expression promoters
Mini promoter
Fluorescent markers
Chemical light reporter markers
Chromogenic reporter markers
PH-sensitive fluorescent reporter markers
Calcium indicator markers
Yeast screening markers
Antibiotics resistance gene
Read-through linker
Non-read-through linker
Dual Reporter gene
Epitope Tags
Purification Tags
Protein cleavage sites
Signal peptides
Degron Tags
Dimer domain
Cell penetrating peptide
BE3
Cas12a
Cas9
dCas9
Chimeric antigen receptor domain
Antibody heavy chain constant region
Antibody light chain constant region
Transcriptional regulatory protein
Tet regulatory protein
DNA Recombinase
Drug DNA recombinase
Regulatory elements
Enhancer elements
Standard elements Library
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Promoter
All
C. elegans constitutive expression promoters
C. elegans inducible expression promoters
Drosophila constitutive expression promoters
Drosophila inducible expression promoters
Drosophila Pol III promoters
Mammalian constitutive expression promoters
Mammalian inducible expression promoters
Mini promoter
Plant constitutive expression promoters
Plant Pol III promoters
Yeast constitutive expression promoters
Yeast inducible expression promoters
Zebrafish constitutive expression promoters
Zebrafish inducible expression promoters
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Description
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Name
Sequence
Description
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All
C. elegans tissue-specific expression promoters
Drosophila tissue-specific promoters
Mammalian tissue-specific expression promoters
All Tissue Specificity
Adult smooth muscle
Blood
Blood vessels
Bone
Bone and cartilage
Bone marrow
Bone, tooth and tendon
Brain
Brain, hippocampus, cerebellum, retina
Cartilage
Embryonic developing pancreas and adult pancreatic islets
Embryonic heart
Embryonic inner cell mass
Epidermis
Fat
Forebrain
Heart
Kidney
Liver
Lung
Lung, heart, ovary, spleen and kidney glomeruli
Mammary gland
Mouse skeletal and heart muscles
Mouse skeletal, heart, and smooth muscle
Muscle
Nervous system
Nervous system at embryonic stage
Nervous systems at embryonic and early postnatal stages
Nervous systems, testis and thymus
Ocelli
Pancreas
Retina
Spinal cord
Tumor
All Cell Type Specificity
Adipocytes
Alveolar epithelial cells of mammary tissue
Astrocytes
AT II cells (alveolar type II epithelial cells) and Clara cells (bronchiolar epithelial cells)
Body muscle cells
CA1 pyramidal cells of the hippocampus
Cardiomyocytes
Chondrocytes
Cone photoreceptor
Cone photoreceptor in non-human primate
Developing neurons but not astrocytes or oligodendrocytes
Differentiated postmitotic striated muscle cells
Dopaminergic neurons
Ductal cells of the salivary gland, mammary epithelial cells
Early osteoblasts
Endothelial cells
Hepatocytes
Immortalized and cancer cells
Keratinocytes
Mature hepatocytes
Mature myeloid cell lines (neutrophils, monocytes and macrophages)
Mature neurons
Melanocytes and melanoma cells
Microglia
Monocytes and macrophages
Motor neurons
Myoblasts
Myocytes
Neural stem/progenitor cells
Neurons
Ocellar photoreceptor cells
Oligodendrocytes and Schwann cells
ON-bipolar cells
Osteoblasts and fibroblasts
Osteoblasts, osteocytes and hypertrophic chondrocytes
Pancreatic progenitor cells and pancreatic β cells
Pancreatic β cells
Pan-neuronal
Pharyngeal muscle cells
Pluripotent stem cells such as embryonic stem cells
Podocytes
Pyramidal neurons
Rod photoreceptor
RPE
Sperm
Various CNS neurons
Various neurons
Vascular endothelial cells
Vascular smooth muscle cells
β cells
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Sequence
Description
Tissue Specificity
Cell Type Specificity
References
Type
Name
Sequence
Description
Tissue Specificity
Cell Type Specificity
References
Type
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Marker genes
Fluorescent markers
All Structure
Dimer
Monomer
Monomer (may form weak dimer)
Monomer (nonaggregating tandem dimer)
Tetramer
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Sequence
Description
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Color
Structure
Maximum Excitation(nm)
Maximum Emission(nm)
Brightness(% of EGFP)
References
Name
Sequence
Description
Application Notes
Color
Structure
Maximum Excitation(nm)
Maximum Emission(nm)
Brightness(% of EGFP)
References
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Chemical light reporter markers
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Name Descending
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Name
Sequence
Description
Max Emission
Application Notes
References
Name
Sequence
Description
Max Emission
Application Notes
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Chromogenic reporter markers
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Name Descending
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Name
Sequence
Description
Application Notes
Substrate
Color
References
Name
Sequence
Description
Application Notes
Substrate
Color
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PH-sensitive fluorescent reporter markers
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Name Descending
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Name
Sequence
Description
Application Notes
References
Name
Sequence
Description
Application Notes
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Calcium indicator markers
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Name Ascending
Name Descending
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Name
Sequence
Description
Application Notes
References
Name
Sequence
Description
Application Notes
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Yeast screening markers
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Name Ascending
Name Descending
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Name
Sequence
Description
Auxotrophic Complementation
Application Notes
References
Name
Sequence
Description
Auxotrophic Complementation
Application Notes
References
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Antibiotics resistance gene
All Selection Drug
Blasticidin
Bleomycin, phleomycin or Zeocin(TM)
G418 or Kanamycin
Geneticin (G418)
Glufosinate (phosphinothricin)
Hygromycin B
Puromycin
All Usage
Positive selection
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Sequence
Description
Selection Drug
Usage
Application Notes
References
Name
Sequence
Description
Selection Drug
Usage
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ORF linker
All
All
Fusion protein linker
2A peptide
Non-read-through linker
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Name Descending
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Name
Sequence
Description
Application Notes
References
Type
Name
Sequence
Description
Application Notes
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Type
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Dual Reporter gene
All Selection Drug
Blasticidin
Bleomycin, phleomycin or Zeocin(TM)
Geneticin (G418)
Hygromycin B
Puromycin
Default Order
Name Ascending
Name Descending
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Name
Sequence
Description
荧光
Selection Drug
Application Notes
References
Name
Sequence
Description
荧光
Selection Drug
Application Notes
References
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Protein Tag
All
Cell penetrating peptide
Degron Tags
Dimer domain
Epitope Tags
Protein cleavage sites
Purification Tags
Signal peptides
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Name Descending
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Name
Sequence
Description
Application Notes
References
Type
Name
Sequence
Description
Application Notes
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Type
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Cas gene
All
BE3
Cas12a
Cas9
dCas9
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Name Descending
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Name
Sequence
Description
Application Notes
PAM Sequence
References
Type
Name
Sequence
Description
Application Notes
PAM Sequence
References
Type
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Antibody associated element
All
Antibody heavy chain constant region
Antibody light chain constant region
Chimeric antigen receptor domain
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Name Descending
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Name
Sequence
Description
Application Notes
References
Type
Name
Sequence
Description
Application Notes
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Expression regulatory gene
All
DNA Recombinase
Drug DNA recombinase
Tet regulatory protein
Transcriptional regulatory protein
All Target Sites
CuO
FRT and its variants (e.g. FRT3, FRT5)
Inverted/direct repeats (IR/DR) of Mos1 transposon
Inverted/direct repeats (IR/DR) of Sleeping Beauty transposon
LacO
LoxP and its variants (e.g. LoxN, Lox2272, Lox511, Lox66, Lox71)
PiggyBac inverted terminal repeat (ITR)
Rox and its variants (e.g. Rox4R, Rox6R, Rox2N )
SunTag
Tol2 invertet terminal repeat (ITR)
TRE
TRE3G
UAS
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Sequence
Description
Application Notes
Target Sites
References
Type
Name
Sequence
Description
Application Notes
Target Sites
References
Type
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Regulatory elements
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Name Descending
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Name
Sequence
Description
Application Notes
References
Name
Sequence
Description
Application Notes
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Enhancer elements
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Sequence
Description
Application Notes
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Name
Sequence
Description
Application Notes
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Standard elements Library
All
5'UTR
CDS
enhancer
exon
gene
intron
LTR
misc_feature
misc_recomb
misc_RNA
misc_signal
mobile_element
oriT
polyA_signal
polyA_site
primer_bind
promoter
protein_bind
rep_origin
repeat_region
terminator
tRNA
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Sequence
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Type
optional
CDS
exon
gap
gene
intron
misc_feature
mRNA
primer_bind
promoter
protein_bind
rep_origin
source
terminator
Description
optional
Setting
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File Format
.gb
.fa
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