CMV enhancer(1-380) CMV enhancer CMV enhancer(1-380) CMV enhancer CMV enhancer(1-380) CMV enh... CMV enhancer(1-380) CMV promoter(382-580) CMV promoter CMV promoter(382-580) CMV promoter CMV promoter(382-580) CMV promoter CMV promoter(382-580) 5' LTR (truncated)(598-778) 5' LTR (truncated) 5' LTR (truncated)(598-778) 5' LTR (truncated) 5' LTR (truncated)(598-778) 5' LTR (truncated) 5' LTR (truncated)(598-778) HIV-1 Ψ(825-950) HIV-1 Ψ HIV-1 Ψ(825-950) HIV-1 Ψ HIV-1 Ψ(825-950) HIV-1 Ψ HIV-1 Ψ(825-950) RRE(1443-1676) RRE RRE(1443-1676) RRE RRE(1443-1676) gp41 peptide(1861-1905) gp41 peptide gp41 peptide(1861-1905) gp41 peptide gp41 peptide(1861-1905) gp41 peptide gp41 peptide(1861-1905) cPPT/CTS(2203-2320) cPPT/CTS cPPT/CTS(2203-2320) cPPT/CTS cPPT/CTS(2203-2320) cPPT/CTS cPPT/CTS(2203-2320) U6 promoter(2371-2611) U6 promoter U6 promoter(2371-2611) U6 promoter U6 promoter(2371-2611) U6 promoter U6 promoter(2371-2611) RfxCas13d DR30(2621-2650) RfxCas13d DR30 RfxCas13d DR30(2621-2650) RfxCas13d DR30 RfxCas13d DR30(2621-2650) RfxCas13d DR30 RfxCas13d DR30(2621-2650) EF-1α core promoter(2729-2940) EF-1α core promoter EF-1α core promoter(2729-2940) EF-1α core promoter EF-1α core promoter(2729-2940) EF-1α core promoter EF-1α core promoter(2729-2940) RfxCas13d(2965-5862) RfxCas13d RfxCas13d(2965-5862) RfxCas13d RfxCas13d(2965-5862) nucleoplasmin NLS(5863-5910) nucleoplasmin NLS nucleoplasmin NLS(5863-5910) nucleoplasmin NLS nucleoplasmin NLS(5863-5910) ...nucleop nucleoplasmin NLS(5863-5910) P2A(5920-5976) P2A P2A(5920-5976) P2A P2A(5920-5976) P2A P2A(5920-5976) PuroR(5977-6573) PuroR PuroR(5977-6573) PuroR PuroR(5977-6573) WPRE(6589-7177) WPRE WPRE(6589-7177) WPRE WPRE(6589-7177) Factor Xa site(7060-7071) Factor Xa site Factor Xa site(7060-7071) Factor Xa site Factor Xa site(7060-7071) ...Factor Factor Xa site(7060-7071) 3' LTR (ΔU3)(7249-7482) 3' LTR (ΔU3) 3' LTR (ΔU3)(7249-7482) 3' LTR (ΔU3) 3' LTR (ΔU3)(7249-7482) 3' LTR (ΔU3) 3' LTR (ΔU3)(7249-7482) bGH poly(A) signal(7514-7738) bGH poly(A) signal bGH poly(A) signal(7514-7738) bGH poly(A) signal bGH poly(A) signal(7514-7738) bGH poly(A) signal bGH poly(A) signal(7514-7738) f1 ori(7784-8212) f1 ori f1 ori(7784-8212) f1 ori f1 ori(7784-8212) f1 ori f1 ori(7784-8212) SV40 promoter(8226-8555) SV40 promoter SV40 promoter(8226-8555) SV40 promoter SV40 promoter(8226-8555) SV40 promoter SV40 promoter(8226-8555) SV40 ori(8406-8541) SV40 ori SV40 ori(8406-8541) SV40 ori SV40 ori(8406-8541) SV40 ori SV40 ori(8406-8541) EM7 promoter(8603-8650) EM7 promoter EM7 promoter(8603-8650) EM7 promoter EM7 promoter(8603-8650) EM7 promoter EM7 promoter(8603-8650) BleoR(8669-9043) BleoR BleoR(8669-9043) BleoR BleoR(8669-9043) SV40 poly(A) signal(9173-9294) SV40 poly(A) signal SV40 poly(A) signal(9173-9294) SV40 poly(A) signal SV40 poly(A) signal(9173-9294) SV40 poly(A) signal SV40 poly(A) signal(9173-9294) M13 rev(9343-9359) M13 rev M13 rev(9343-9359) M13 rev M13 rev(9343-9359) M13 rev M13 rev(9343-9359) lac operator(9367-9383) lac operator lac operator(9367-9383) lac operator lac operator(9367-9383) lac operator lac operator(9367-9383) lac promoter(9391-9421) lac promoter lac promoter(9391-9421) lac promoter lac promoter(9391-9421) lac promoter lac promoter(9391-9421) CAP binding site(9436-9457) CAP binding site CAP binding site(9436-9457) CAP binding site CAP binding site(9436-9457) CAP binding site CAP binding site(9436-9457) ori(9745-10333) ori ori(9745-10333) ori ori(9745-10333) AmpR(10504-11364) AmpR AmpR(10504-11364) AmpR AmpR(10504-11364) AmpR promoter(11365-11469) AmpR promoter AmpR promoter(11365-11469) AmpR promoter AmpR promoter(11365-11469) AmpR promoter AmpR promoter(11365-11469) Factor Xa site(7062-7073) Factor Xa site Factor Xa site(7062-7073) Factor Xa site Factor Xa site(7062-7073) ...Factor Factor Xa site(7062-7073) M13 rev(9345-9361) M13 rev M13 rev(9345-9361) M13 rev M13 rev(9345-9361) M13 rev M13 rev(9345-9361) lac operator(9369-9385) lac operator lac operator(9369-9385) lac operator lac operator(9369-9385) lac operator lac operator(9369-9385) CAP binding site(9438-9459) CAP binding site CAP binding site(9438-9459) CAP binding site CAP binding site(9438-9459) CAP binding site CAP binding site(9438-9459) lac promoter(9393-9423) lac promoter lac promoter(9393-9423) lac promoter lac promoter(9393-9423) lac promoter lac promoter(9393-9423) EM7 promoter(8605-8652) EM7 promoter EM7 promoter(8605-8652) EM7 promoter EM7 promoter(8605-8652) EM7 promoter EM7 promoter(8605-8652) AmpR promoter(11367-11471) AmpR promoter AmpR promoter(11367-11471) AmpR promoter AmpR promoter(11367-11471) AmpR promoter AmpR promoter(11367-11471) cPPT/CTS(2205-2322) cPPT/CTS cPPT/CTS(2205-2322) cPPT/CTS cPPT/CTS(2205-2322) cPPT/CTS cPPT/CTS(2205-2322) HIV-1 Ψ(827-952) HIV-1 Ψ HIV-1 Ψ(827-952) HIV-1 Ψ HIV-1 Ψ(827-952) HIV-1 Ψ HIV-1 Ψ(827-952) bGH poly(A) signal(7516-7740) bGH poly(A) signal bGH poly(A) signal(7516-7740) bGH poly(A) signal bGH poly(A) signal(7516-7740) ...bGH pol bGH poly(A) signal(7516-7740) RRE(1445-1678) RRE RRE(1445-1678) RRE RRE(1445-1678) RRE RRE(1445-1678) 3' LTR (ΔU3)(7251-7484) 3' LTR (ΔU3) 3' LTR (ΔU3)(7251-7484) 3' LTR (ΔU3) 3' LTR (ΔU3)(7251-7484) 3' LTR (ΔU3) 3' LTR (ΔU3)(7251-7484) BleoR(8671-9045) BleoR BleoR(8671-9045) BleoR BleoR(8671-9045) BleoR BleoR(8671-9045) CMV enhancer(3-382) CMV enhancer CMV enhancer(3-382) CMV enhancer CMV enhancer(3-382) CMV enhancer CMV enhancer(3-382) WPRE(6591-7179) WPRE WPRE(6591-7179) WPRE WPRE(6591-7179)