CMV enhancer(254-633) CMV enhancer CMV enhancer(254-633) CMV enhancer CMV enhancer(254-633) CMV enh... CMV enhancer(254-633) CMV promoter(635-833) CMV promoter CMV promoter(635-833) CMV promoter CMV promoter(635-833) CMV promoter CMV promoter(635-833) 5' LTR (truncated)(851-1031) 5' LTR (truncated) 5' LTR (truncated)(851-1031) 5' LTR (truncated) 5' LTR (truncated)(851-1031) 5' LTR ... 5' LTR (truncated)(851-1031) HIV-1 Ψ(1078-1203) HIV-1 Ψ HIV-1 Ψ(1078-1203) HIV-1 Ψ HIV-1 Ψ(1078-1203) HIV-1 Ψ HIV-1 Ψ(1078-1203) RRE(1696-1929) RRE RRE(1696-1929) RRE RRE(1696-1929) gp41 peptide(2114-2158) gp41 peptide gp41 peptide(2114-2158) gp41 peptide gp41 peptide(2114-2158) gp41 peptide gp41 peptide(2114-2158) cPPT/CTS(2456-2573) cPPT/CTS cPPT/CTS(2456-2573) cPPT/CTS cPPT/CTS(2456-2573) cPPT/CTS cPPT/CTS(2456-2573) U6 promoter(2624-2864) U6 promoter U6 promoter(2624-2864) U6 promoter U6 promoter(2624-2864) U6 promoter U6 promoter(2624-2864) RfxCas13d DR30(2874-2903) RfxCas13d DR30 RfxCas13d DR30(2874-2903) RfxCas13d DR30 RfxCas13d DR30(2874-2903) RfxCas13d DR30 RfxCas13d DR30(2874-2903) EF-1α core promoter(2980-3191) EF-1α core promoter EF-1α core promoter(2980-3191) EF-1α core promoter EF-1α core promoter(2980-3191) EF-1α core promoter EF-1α core promoter(2980-3191) PuroR(3207-3806) PuroR PuroR(3207-3806) PuroR PuroR(3207-3806) WPRE(3822-4410) WPRE WPRE(3822-4410) WPRE WPRE(3822-4410) Factor Xa site(4293-4304) Factor Xa site Factor Xa site(4293-4304) Factor Xa site Factor Xa site(4293-4304) Factor Xa site Factor Xa site(4293-4304) 3' LTR (ΔU3)(4482-4715) 3' LTR (ΔU3) 3' LTR (ΔU3)(4482-4715) 3' LTR (ΔU3) 3' LTR (ΔU3)(4482-4715) 3' LTR (ΔU3) 3' LTR (ΔU3)(4482-4715) bGH poly(A) signal(4747-4971) bGH poly(A) signal bGH poly(A) signal(4747-4971) bGH poly(A) signal bGH poly(A) signal(4747-4971) bGH poly(A) signal bGH poly(A) signal(4747-4971) f1 ori(5017-5445) f1 ori f1 ori(5017-5445) f1 ori f1 ori(5017-5445) SV40 promoter(5459-5788) SV40 promoter SV40 promoter(5459-5788) SV40 promoter SV40 promoter(5459-5788) SV40 promoter SV40 promoter(5459-5788) SV40 ori(5639-5774) SV40 ori SV40 ori(5639-5774) SV40 ori SV40 ori(5639-5774) SV40 ori SV40 ori(5639-5774) EM7 promoter(5836-5883) EM7 promoter EM7 promoter(5836-5883) EM7 promoter EM7 promoter(5836-5883) EM7 promoter EM7 promoter(5836-5883) BleoR(5902-6276) BleoR BleoR(5902-6276) BleoR BleoR(5902-6276) SV40 poly(A) signal(6406-6527) SV40 poly(A) signal SV40 poly(A) signal(6406-6527) SV40 poly(A) signal SV40 poly(A) signal(6406-6527) SV40 poly(A) signal SV40 poly(A) signal(6406-6527) M13 rev(6576-6592) M13 rev M13 rev(6576-6592) M13 rev M13 rev(6576-6592) M13 rev M13 rev(6576-6592) lac operator(6600-6616) lac operator lac operator(6600-6616) lac operator lac operator(6600-6616) lac operator lac operator(6600-6616) lac promoter(6624-6654) lac promoter lac promoter(6624-6654) lac promoter lac promoter(6624-6654) lac promoter lac promoter(6624-6654) CAP binding site(6669-6690) CAP binding site CAP binding site(6669-6690) CAP binding site CAP binding site(6669-6690) CAP binding site CAP binding site(6669-6690) ori(6978-7566) ori ori(6978-7566) ori ori(6978-7566) AmpR(7737-8597) AmpR AmpR(7737-8597) AmpR AmpR(7737-8597) AmpR promoter(8598-8702) AmpR promoter AmpR promoter(8598-8702) AmpR promoter AmpR promoter(8598-8702) AmpR promoter AmpR promoter(8598-8702) Factor Xa site(4295-4306) Factor Xa site Factor Xa site(4295-4306) Factor Xa site Factor Xa site(4295-4306) Factor Xa site Factor Xa site(4295-4306) M13 rev(6578-6594) M13 rev M13 rev(6578-6594) M13 rev M13 rev(6578-6594) M13 rev M13 rev(6578-6594) lac operator(6602-6618) lac operator lac operator(6602-6618) lac operator lac operator(6602-6618) lac operator lac operator(6602-6618) CAP binding site(6671-6692) CAP binding site CAP binding site(6671-6692) CAP binding site CAP binding site(6671-6692) CAP binding site CAP binding site(6671-6692) lac promoter(6626-6656) lac promoter lac promoter(6626-6656) lac promoter lac promoter(6626-6656) lac promoter lac promoter(6626-6656) EM7 promoter(5838-5885) EM7 promoter EM7 promoter(5838-5885) EM7 promoter EM7 promoter(5838-5885) EM7 promoter EM7 promoter(5838-5885) AmpR promoter(8600-8704) AmpR promoter AmpR promoter(8600-8704) AmpR promoter AmpR promoter(8600-8704) AmpR promoter AmpR promoter(8600-8704) cPPT/CTS(2458-2575) cPPT/CTS cPPT/CTS(2458-2575) cPPT/CTS cPPT/CTS(2458-2575) cPPT/CTS cPPT/CTS(2458-2575) HIV-1 Ψ(1080-1205) HIV-1 Ψ HIV-1 Ψ(1080-1205) HIV-1 Ψ HIV-1 Ψ(1080-1205) HIV-1 Ψ HIV-1 Ψ(1080-1205) bGH poly(A) signal(4749-4973) bGH poly(A) signal bGH poly(A) signal(4749-4973) bGH poly(A) signal bGH poly(A) signal(4749-4973) bGH poly(A) signal bGH poly(A) signal(4749-4973) RRE(1698-1931) RRE RRE(1698-1931) RRE RRE(1698-1931) 3' LTR (ΔU3)(4484-4717) 3' LTR (ΔU3) 3' LTR (ΔU3)(4484-4717) 3' LTR (ΔU3) 3' LTR (ΔU3)(4484-4717) 3' LTR (ΔU3) 3' LTR (ΔU3)(4484-4717) BleoR(5904-6278) BleoR BleoR(5904-6278) BleoR BleoR(5904-6278) CMV enhancer(256-635) CMV enhancer CMV enhancer(256-635) CMV enhancer CMV enhancer(256-635) CMV enhancer CMV enhancer(256-635) WPRE(3824-4412) WPRE WPRE(3824-4412) WPRE WPRE(3824-4412)