HIV-1 Ψ(481-606) HIV-1 Ψ HIV-1 Ψ(481-606) HIV-1 Ψ HIV-1 Ψ(481-606) HIV-1 Ψ HIV-1 Ψ(481-606) 5' LTR (truncated)(653-833) 5' LTR (truncated) 5' LTR (truncated)(653-833) 5' LTR (truncated) 5' LTR (truncated)(653-833) 5' LTR (truncated) 5' LTR (truncated)(653-833) CMV promoter(851-1049) CMV promoter CMV promoter(851-1049) CMV promoter CMV promoter(851-1049) CMV promoter CMV promoter(851-1049) CMV enhancer(1051-1430) CMV enhancer CMV enhancer(1051-1430) CMV enhancer CMV enhancer(1051-1430) CMV enhancer CMV enhancer(1051-1430) AmpR promoter(1696-1800) AmpR promoter AmpR promoter(1696-1800) AmpR promoter AmpR promoter(1696-1800) AmpR promoter AmpR promoter(1696-1800) AmpR(1801-2661) AmpR AmpR(1801-2661) AmpR AmpR(1801-2661) ori(2832-3420) ori ori(2832-3420) ori ori(2832-3420) CAP binding site(3708-3729) CAP binding site CAP binding site(3708-3729) CAP binding site CAP binding site(3708-3729) CAP binding site CAP binding site(3708-3729) lac promoter(3744-3774) lac promoter lac promoter(3744-3774) lac promoter lac promoter(3744-3774) lac promoter lac promoter(3744-3774) lac operator(3782-3798) lac operator lac operator(3782-3798) lac operator lac operator(3782-3798) lac ope... lac operator(3782-3798) M13 rev(3806-3822) M13 rev M13 rev(3806-3822) M13 rev M13 rev(3806-3822) M13 rev M13 rev(3806-3822) SV40 poly(A) signal(3871-3992) SV40 poly(A) signal SV40 poly(A) signal(3871-3992) SV40 poly(A) signal SV40 poly(A) signal(3871-3992) SV40 poly(A) signal SV40 poly(A) signal(3871-3992) BleoR(4122-4496) BleoR BleoR(4122-4496) BleoR BleoR(4122-4496) EM7 promoter(4515-4562) EM7 promoter EM7 promoter(4515-4562) EM7 promoter EM7 promoter(4515-4562) EM7 pro... EM7 promoter(4515-4562) SV40 ori(4624-4759) SV40 ori SV40 ori(4624-4759) SV40 ori SV40 ori(4624-4759) SV40 or... SV40 ori(4624-4759) SV40 promoter(4610-4939) SV40 promoter SV40 promoter(4610-4939) SV40 promoter SV40 promoter(4610-4939) SV40 pr... SV40 promoter(4610-4939) f1 ori(4953-5381) f1 ori f1 ori(4953-5381) f1 ori f1 ori(4953-5381) f1 ori f1 ori(4953-5381) bGH poly(A) signal(5427-5651) bGH poly(A) signal bGH poly(A) signal(5427-5651) bGH poly(A) signal bGH poly(A) signal(5427-5651) bGH pol... bGH poly(A) signal(5427-5651) 5' LTR (truncated)(5683-5863) 5' LTR (truncated) 5' LTR (truncated)(5683-5863) 5' LTR (truncated) 5' LTR (truncated)(5683-5863) 5' LTR (truncated) 5' LTR (truncated)(5683-5863) KS primer(6369-6385) KS primer KS primer(6369-6385) KS primer KS primer(6369-6385) KS primer KS primer(6369-6385) EF-1α promoter(6468-7646) EF-1α promoter EF-1α promoter(6468-7646) EF-1α promoter EF-1α promoter(6468-7646) EF-1α intron A(6699-7637) EF-1α intron A EF-1α intron A(6699-7637) EF-1α intron A EF-1α intron A(6699-7637) EF-1α intron A EF-1α intron A(6699-7637) Kozak sequence(7653-7662) Kozak sequence Kozak sequence(7653-7662) Kozak sequence Kozak sequence(7653-7662) Kozak sequence Kozak sequence(7653-7662) MS2-N55K(7662-8048) MS2-N55K MS2-N55K(7662-8048) MS2-N55K MS2-N55K(7662-8048) MS2-N55K MS2-N55K(7662-8048) SV40 NLS(8103-8123) SV40 NLS SV40 NLS(8103-8123) SV40 NLS SV40 NLS(8103-8123) SV40 NLS SV40 NLS(8103-8123) p65(8139-8681) p65 p65(8139-8681) p65 p65(8139-8681) HSF1(8706-9077) HSF1 HSF1(8706-9077) HSF1 HSF1(8706-9077) T2A(9093-9146) T2A T2A(9093-9146) T2A T2A(9093-9146) T2A T2A(9093-9146) HygR(9150-10169) HygR HygR(9150-10169) HygR HygR(9150-10169) WPRE(10209-10797) WPRE WPRE(10209-10797) WPRE WPRE(10209-10797) Factor Xa site(10680-10691) Factor Xa site Factor Xa site(10680-10691) Factor Xa site Factor Xa site(10680-10691) Factor Xa site Factor Xa site(10680-10691) cPPT/CTS(10850-10967) cPPT/CTS cPPT/CTS(10850-10967) cPPT/CTS cPPT/CTS(10850-10967) ...cPPT/CT cPPT/CTS(10850-10967) gp41 peptide(11265-11309) gp41 peptide gp41 peptide(11265-11309) gp41 peptide gp41 peptide(11265-11309) ...gp41 pe gp41 peptide(11265-11309) RRE(11494-11727) RRE RRE(11494-11727) RRE RRE(11494-11727) Factor Xa site(10682-10693) Factor Xa site Factor Xa site(10682-10693) Factor Xa site Factor Xa site(10682-10693) ...Factor Factor Xa site(10682-10693) KS primer(6371-6387) KS primer KS primer(6371-6387) KS primer KS primer(6371-6387) KS primer KS primer(6371-6387) M13 rev(3808-3824) M13 rev M13 rev(3808-3824) M13 rev M13 rev(3808-3824) M13 rev M13 rev(3808-3824) lac operator(3784-3800) lac operator lac operator(3784-3800) lac operator lac operator(3784-3800) lac ope... lac operator(3784-3800) CAP binding site(3710-3731) CAP binding site CAP binding site(3710-3731) CAP binding site CAP binding site(3710-3731) CAP binding site CAP binding site(3710-3731) lac promoter(3746-3776) lac promoter lac promoter(3746-3776) lac promoter lac promoter(3746-3776) lac pro... lac promoter(3746-3776) EM7 promoter(4517-4564) EM7 promoter EM7 promoter(4517-4564) EM7 promoter EM7 promoter(4517-4564) EM7 pro... EM7 promoter(4517-4564) AmpR promoter(1698-1802) AmpR promoter AmpR promoter(1698-1802) AmpR promoter AmpR promoter(1698-1802) AmpR promoter AmpR promoter(1698-1802) cPPT/CTS(10852-10969) cPPT/CTS cPPT/CTS(10852-10969) cPPT/CTS cPPT/CTS(10852-10969) cPPT/CTS cPPT/CTS(10852-10969) HIV-1 Ψ(483-608) HIV-1 Ψ HIV-1 Ψ(483-608) HIV-1 Ψ HIV-1 Ψ(483-608) HIV-1 Ψ HIV-1 Ψ(483-608) bGH poly(A) signal(5429-5653) bGH poly(A) signal bGH poly(A) signal(5429-5653) bGH poly(A) signal bGH poly(A) signal(5429-5653) bGH pol... bGH poly(A) signal(5429-5653) RRE(11496-11729) RRE RRE(11496-11729) RRE RRE(11496-11729) RRE RRE(11496-11729) BleoR(4124-4498) BleoR BleoR(4124-4498) BleoR BleoR(4124-4498) BleoR BleoR(4124-4498) CMV enhancer(1053-1432) CMV enhancer CMV enhancer(1053-1432) CMV enhancer CMV enhancer(1053-1432) CMV enhancer CMV enhancer(1053-1432) WPRE(10211-10799) WPRE WPRE(10211-10799) WPRE WPRE(10211-10799)