CMV promoter(1-199) CMV promoter CMV promoter(1-199) CMV promoter CMV promoter(1-199) CMV pro... CMV promoter(1-199) 5' LTR (truncated)(217-397) 5' LTR (truncated) 5' LTR (truncated)(217-397) 5' LTR (truncated) 5' LTR (truncated)(217-397) 5' LTR ... 5' LTR (truncated)(217-397) HIV-1 Ψ(444-569) HIV-1 Ψ HIV-1 Ψ(444-569) HIV-1 Ψ HIV-1 Ψ(444-569) HIV-1 Ψ HIV-1 Ψ(444-569) RRE(1062-1295) RRE RRE(1062-1295) RRE RRE(1062-1295) RRE RRE(1062-1295) gp41 peptide(1480-1524) gp41 peptide gp41 peptide(1480-1524) gp41 peptide gp41 peptide(1480-1524) gp41 pe... gp41 peptide(1480-1524) cPPT/CTS(1822-1939) cPPT/CTS cPPT/CTS(1822-1939) cPPT/CTS cPPT/CTS(1822-1939) cPPT/CTS cPPT/CTS(1822-1939) gRNA scaffold(2071-2146) gRNA scaffold gRNA scaffold(2071-2146) gRNA scaffold gRNA scaffold(2071-2146) gRNA scaffold gRNA scaffold(2071-2146) EF-1α core promoter(2208-2419) EF-1α core promoter EF-1α core promoter(2208-2419) EF-1α core promoter EF-1α core promoter(2208-2419) EF-1α core promoter EF-1α core promoter(2208-2419) AsCpf1(2444-6364) AsCpf1 AsCpf1(2444-6364) AsCpf1 AsCpf1(2444-6364) Kozak sequence(6084-6093) Kozak sequence Kozak sequence(6084-6093) Kozak sequence Kozak sequence(6084-6093) Kozak sequence Kozak sequence(6084-6093) nucleoplasmin NLS(6365-6412) nucleoplasmin NLS nucleoplasmin NLS(6365-6412) nucleoplasmin NLS nucleoplasmin NLS(6365-6412) nucleoplasmin NLS nucleoplasmin NLS(6365-6412) FLAG(6413-6436) FLAG FLAG(6413-6436) FLAG FLAG(6413-6436) FLAG FLAG(6413-6436) attB1(6479-6503) attB1 attB1(6479-6503) attB1 attB1(6479-6503) attB1 attB1(6479-6503) VP64(6533-6682) VP64 VP64(6533-6682) VP64 VP64(6533-6682) VP64 VP64(6533-6682) SV40 NLS(6707-6727) SV40 NLS SV40 NLS(6707-6727) SV40 NLS SV40 NLS(6707-6727) SV40 NLS SV40 NLS(6707-6727) RelA (p65) AD(7157-7513) RelA (p65) AD RelA (p65) AD(7157-7513) RelA (p65) AD RelA (p65) AD(7157-7513) RelA (p65) AD RelA (p65) AD(7157-7513) Rta AD(7532-8101) Rta AD Rta AD(7532-8101) Rta AD Rta AD(7532-8101) P2A(8111-8167) P2A P2A(8111-8167) P2A P2A(8111-8167) P2A P2A(8111-8167) PuroR(8168-8764) PuroR PuroR(8168-8764) PuroR PuroR(8168-8764) WPRE(8780-9368) WPRE WPRE(8780-9368) WPRE WPRE(8780-9368) Factor Xa site(9251-9262) Factor Xa site Factor Xa site(9251-9262) Factor Xa site Factor Xa site(9251-9262) Factor Xa site Factor Xa site(9251-9262) 3' LTR (ΔU3)(9440-9673) 3' LTR (ΔU3) 3' LTR (ΔU3)(9440-9673) 3' LTR (ΔU3) 3' LTR (ΔU3)(9440-9673) 3' LTR (ΔU3) 3' LTR (ΔU3)(9440-9673) bGH poly(A) signal(9705-9929) bGH poly(A) signal bGH poly(A) signal(9705-9929) bGH poly(A) signal bGH poly(A) signal(9705-9929) bGH poly(A) signal bGH poly(A) signal(9705-9929) f1 ori(9975-10403) f1 ori f1 ori(9975-10403) f1 ori f1 ori(9975-10403) f1 ori f1 ori(9975-10403) SV40 promoter(10417-10746) SV40 promoter SV40 promoter(10417-10746) SV40 promoter SV40 promoter(10417-10746) SV40 promoter SV40 promoter(10417-10746) SV40 ori(10597-10732) SV40 ori SV40 ori(10597-10732) SV40 ori SV40 ori(10597-10732) SV40 ori SV40 ori(10597-10732) EM7 promoter(10794-10841) EM7 promoter EM7 promoter(10794-10841) EM7 promoter EM7 promoter(10794-10841) EM7 promoter EM7 promoter(10794-10841) BleoR(10860-11234) BleoR BleoR(10860-11234) BleoR BleoR(10860-11234) BleoR BleoR(10860-11234) SV40 poly(A) signal(11364-11485) SV40 poly(A) signal SV40 poly(A) signal(11364-11485) SV40 poly(A) signal SV40 poly(A) signal(11364-11485) SV40 poly(A) signal SV40 poly(A) signal(11364-11485) M13 rev(11534-11550) M13 rev M13 rev(11534-11550) M13 rev M13 rev(11534-11550) M13 rev M13 rev(11534-11550) lac operator(11558-11574) lac operator lac operator(11558-11574) lac operator lac operator(11558-11574) lac operator lac operator(11558-11574) lac promoter(11582-11612) lac promoter lac promoter(11582-11612) lac promoter lac promoter(11582-11612) lac promoter lac promoter(11582-11612) CAP binding site(11627-11648) CAP binding site CAP binding site(11627-11648) CAP binding site CAP binding site(11627-11648) CAP binding site CAP binding site(11627-11648) ori(11936-12524) ori ori(11936-12524) ori ori(11936-12524) AmpR(12695-13555) AmpR AmpR(12695-13555) AmpR AmpR(12695-13555) AmpR promoter(13556-13660) AmpR promoter AmpR promoter(13556-13660) AmpR promoter AmpR promoter(13556-13660) AmpR promoter AmpR promoter(13556-13660) CMV enhancer(13926-14305) CMV enhancer CMV enhancer(13926-14305) CMV enhancer CMV enhancer(13926-14305) CMV enhancer CMV enhancer(13926-14305) Factor Xa site(9253-9264) Factor Xa site Factor Xa site(9253-9264) Factor Xa site Factor Xa site(9253-9264) Factor Xa site Factor Xa site(9253-9264) M13 rev(11536-11552) M13 rev M13 rev(11536-11552) M13 rev M13 rev(11536-11552) M13 rev M13 rev(11536-11552) lac operator(11560-11576) lac operator lac operator(11560-11576) lac operator lac operator(11560-11576) lac operator lac operator(11560-11576) attB1(6482-6502) attB1 attB1(6482-6502) attB1 attB1(6482-6502) attB1 attB1(6482-6502) CAP binding site(11629-11650) CAP binding site CAP binding site(11629-11650) CAP binding site CAP binding site(11629-11650) CAP binding site CAP binding site(11629-11650) lac promoter(11584-11614) lac promoter lac promoter(11584-11614) lac promoter lac promoter(11584-11614) lac promoter lac promoter(11584-11614) EM7 promoter(10796-10843) EM7 promoter EM7 promoter(10796-10843) EM7 promoter EM7 promoter(10796-10843) EM7 promoter EM7 promoter(10796-10843) gRNA scaffold(2073-2148) gRNA scaffold gRNA scaffold(2073-2148) gRNA scaffold gRNA scaffold(2073-2148) gRNA scaffold gRNA scaffold(2073-2148) AmpR promoter(13558-13662) AmpR promoter AmpR promoter(13558-13662) AmpR promoter AmpR promoter(13558-13662) AmpR promoter AmpR promoter(13558-13662) cPPT/CTS(1824-1941) cPPT/CTS cPPT/CTS(1824-1941) cPPT/CTS cPPT/CTS(1824-1941) cPPT/CTS cPPT/CTS(1824-1941) HIV-1 Ψ(446-571) HIV-1 Ψ HIV-1 Ψ(446-571) HIV-1 Ψ HIV-1 Ψ(446-571) HIV-1 Ψ HIV-1 Ψ(446-571) bGH poly(A) signal(9707-9931) bGH poly(A) signal bGH poly(A) signal(9707-9931) bGH poly(A) signal bGH poly(A) signal(9707-9931) bGH poly(A) signal bGH poly(A) signal(9707-9931) RRE(1064-1297) RRE RRE(1064-1297) RRE RRE(1064-1297) RRE RRE(1064-1297) 3' LTR (ΔU3)(9442-9675) 3' LTR (ΔU3) 3' LTR (ΔU3)(9442-9675) 3' LTR (ΔU3) 3' LTR (ΔU3)(9442-9675) 3' LTR (ΔU3) 3' LTR (ΔU3)(9442-9675) BleoR(10862-11236) BleoR BleoR(10862-11236) BleoR BleoR(10862-11236) BleoR BleoR(10862-11236) CMV enhancer(13928-1) CMV enhancer CMV enhancer(13928-1) CMV enhancer CMV enhancer(13928-1) CMV enhancer CMV enhancer(13928-1) WPRE(8782-9370) WPRE WPRE(8782-9370) WPRE WPRE(8782-9370)