Design Vector
Expression regulatory vector-Mammal-GIm03
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3xFLAG(463-528)
3xFLAG
3xFLAG(463-528)
3xFLAG
3xFLAG(463-528)
3xFLAG
3xFLAG(463-528)
SV40 NLS(535-555)
SV40 NLS
SV40 NLS(535-555)
SV40 NLS
SV40 NLS(535-555)
SV40 NLS
SV40 NLS(535-555)
Factor Xa site(3152-3163)
Factor Xa site
Factor Xa site(3152-3163)
Factor Xa site
Factor Xa site(3152-3163)
Factor Xa site
Factor Xa site(3152-3163)
SV40 NLS(4681-4701)
SV40 NLS
SV40 NLS(4681-4701)
SV40 NLS
SV40 NLS(4681-4701)
SV40 NLS
SV40 NLS(4681-4701)
SV40 NLS(4996-5016)
SV40 NLS
SV40 NLS(4996-5016)
SV40 NLS
SV40 NLS(4996-5016)
SV40 NLS
SV40 NLS(4996-5016)
PuroR(5077-5676)
PuroR
PuroR(5077-5676)
PuroR
PuroR(5077-5676)
PuroR
PuroR(5077-5676)
loxP(5730-5763)
loxP
loxP(5730-5763)
loxP
loxP(5730-5763)
loxP
loxP(5730-5763)
WPRE(5788-6376)
WPRE
WPRE(5788-6376)
WPRE
WPRE(5788-6376)
WPRE
WPRE(5788-6376)
Factor Xa site(6259-6270)
Factor Xa site
Factor Xa site(6259-6270)
Factor Xa site
Factor Xa site(6259-6270)
Factor ...
Factor Xa site(6259-6270)
KS primer(6379-6395)
KS primer
KS primer(6379-6395)
KS primer
KS primer(6379-6395)
KS prim...
KS primer(6379-6395)
5' LTR(6901-7081)
5' LTR
5' LTR(6901-7081)
5' LTR
5' LTR(6901-7081)
5' LTR
5' LTR(6901-7081)
bGH poly(A) signal(7113-7337)
bGH poly(A) signal
bGH poly(A) signal(7113-7337)
bGH poly(A) signal
bGH poly(A) signal(7113-7337)
bGH pol...
bGH poly(A) signal(7113-7337)
f1 ori(7383-7811)
f1 ori
f1 ori(7383-7811)
f1 ori
f1 ori(7383-7811)
f1 ori
f1 ori(7383-7811)
SV40 promoter(7825-8154)
SV40 promoter
SV40 promoter(7825-8154)
SV40 promoter
SV40 promoter(7825-8154)
...SV40 pr
SV40 promoter(7825-8154)
SV40 ori(8005-8140)
SV40 ori
SV40 ori(8005-8140)
SV40 ori
SV40 ori(8005-8140)
SV40 ori
SV40 ori(8005-8140)
EM7 promoter(8202-8249)
EM7 promoter
EM7 promoter(8202-8249)
EM7 promoter
EM7 promoter(8202-8249)
...EM7 pro
EM7 promoter(8202-8249)
BleoR(8268-8642)
BleoR
BleoR(8268-8642)
BleoR
BleoR(8268-8642)
BleoR
BleoR(8268-8642)
SV40 poly(A) signal(8772-8893)
SV40 poly(A) signal
SV40 poly(A) signal(8772-8893)
SV40 poly(A) signal
SV40 poly(A) signal(8772-8893)
...SV40 po
SV40 poly(A) signal(8772-8893)
M13 rev(8942-8958)
M13 rev
M13 rev(8942-8958)
M13 rev
M13 rev(8942-8958)
M13 rev
M13 rev(8942-8958)
lac operator(8966-8982)
lac operator
lac operator(8966-8982)
lac operator
lac operator(8966-8982)
...lac ope
lac operator(8966-8982)
lac promoter(8990-9020)
lac promoter
lac promoter(8990-9020)
lac promoter
lac promoter(8990-9020)
lac promoter
lac promoter(8990-9020)
ori(9344-9932)
ori
ori(9344-9932)
ori
ori(9344-9932)
ori
ori(9344-9932)
AmpR(10103-10963)
AmpR
AmpR(10103-10963)
AmpR
AmpR(10103-10963)
AmpR
AmpR(10103-10963)
AmpR promoter(10964-11068)
AmpR promoter
AmpR promoter(10964-11068)
AmpR promoter
AmpR promoter(10964-11068)
AmpR promoter
AmpR promoter(10964-11068)
CMV enhancer(11334-11713)
CMV enhancer
CMV enhancer(11334-11713)
CMV enhancer
CMV enhancer(11334-11713)
CMV enhancer
CMV enhancer(11334-11713)
5' LTR(11931-12111)
5' LTR
5' LTR(11931-12111)
5' LTR
5' LTR(11931-12111)
5' LTR
5' LTR(11931-12111)
HIV-1 ψ(12158-12283)
HIV-1 ψ
HIV-1 ψ(12158-12283)
HIV-1 ψ
HIV-1 ψ(12158-12283)
HIV-1 ψ
HIV-1 ψ(12158-12283)
HIV-1 RRE(12776-13009)
HIV-1 RRE
HIV-1 RRE(12776-13009)
HIV-1 RRE
HIV-1 RRE(12776-13009)
HIV-1 RRE
HIV-1 RRE(12776-13009)
HIV-1 cPPT(13536-13653)
HIV-1 cPPT
HIV-1 cPPT(13536-13653)
HIV-1 cPPT
HIV-1 cPPT(13536-13653)
...HIV-1 c
HIV-1 cPPT(13536-13653)
loxP(13697-13730)
loxP
loxP(13697-13730)
loxP
loxP(13697-13730)
loxP
loxP(13697-13730)
Factor Xa site(6261-6272)
Factor Xa site
Factor Xa site(6261-6272)
Factor Xa site
Factor Xa site(6261-6272)
Factor ...
Factor Xa site(6261-6272)
enterokinase site(516-530)
enterokinase site
enterokinase site(516-530)
enterokinase site
enterokinase site(516-530)
enterokinase site
enterokinase site(516-530)
KS primer(6381-6397)
KS primer
KS primer(6381-6397)
KS primer
KS primer(6381-6397)
KS primer
KS primer(6381-6397)
M13 rev(8944-8960)
M13 rev
M13 rev(8944-8960)
M13 rev
M13 rev(8944-8960)
M13 rev
M13 rev(8944-8960)
lac operator(8968-8984)
lac operator
lac operator(8968-8984)
lac operator
lac operator(8968-8984)
...lac ope
lac operator(8968-8984)
CAP binding site(9037-9058)
CAP binding site
CAP binding site(9037-9058)
CAP binding site
CAP binding site(9037-9058)
CAP binding site
CAP binding site(9037-9058)
lac promoter(8992-9022)
lac promoter
lac promoter(8992-9022)
lac promoter
lac promoter(8992-9022)
lac promoter
lac promoter(8992-9022)
loxP(5732-5765)
loxP
loxP(5732-5765)
loxP
loxP(5732-5765)
loxP
loxP(5732-5765)
loxP(13699-13732)
loxP
loxP(13699-13732)
loxP
loxP(13699-13732)
loxP
loxP(13699-13732)
EM7 promoter(8204-8251)
EM7 promoter
EM7 promoter(8204-8251)
EM7 promoter
EM7 promoter(8204-8251)
...EM7 pro
EM7 promoter(8204-8251)
gRNA scaffold(13743-13818)
gRNA scaffold
gRNA scaffold(13743-13818)
gRNA scaffold
gRNA scaffold(13743-13818)
gRNA scaffold
gRNA scaffold(13743-13818)
AmpR promoter(10966-11070)
AmpR promoter
AmpR promoter(10966-11070)
AmpR promoter
AmpR promoter(10966-11070)
AmpR promoter
AmpR promoter(10966-11070)
cPPT/CTS(13538-13655)
cPPT/CTS
cPPT/CTS(13538-13655)
cPPT/CTS
cPPT/CTS(13538-13655)
cPPT/CTS
cPPT/CTS(13538-13655)
HIV-1 Ψ(12160-12285)
HIV-1 Ψ
HIV-1 Ψ(12160-12285)
HIV-1 Ψ
HIV-1 Ψ(12160-12285)
HIV-1 Ψ
HIV-1 Ψ(12160-12285)
bGH poly(A) signal(7115-7339)
bGH poly(A) signal
bGH poly(A) signal(7115-7339)
bGH poly(A) signal
bGH poly(A) signal(7115-7339)
bGH pol...
bGH poly(A) signal(7115-7339)
RRE(12778-13011)
RRE
RRE(12778-13011)
RRE
RRE(12778-13011)
RRE
RRE(12778-13011)
BleoR(8270-8644)
BleoR
BleoR(8270-8644)
BleoR
BleoR(8270-8644)
BleoR
BleoR(8270-8644)
CMV enhancer(11336-11715)
CMV enhancer
CMV enhancer(11336-11715)
CMV enhancer
CMV enhancer(11336-11715)
CMV enhancer
CMV enhancer(11336-11715)
WPRE(5790-6378)
WPRE
WPRE(5790-6378)
WPRE
WPRE(5790-6378)
WPRE
WPRE(5790-6378)
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Select from database
Mammalian constitutive expression promoters
Mammalian tissue-specific expression promoters
Mammalian inducible expression promoters
Zebrafish constitutive expression promoters
Zebrafish inducible expression promoters
Plant constitutive expression promoters
Yeast constitutive expression promoters
Yeast inducible expression promoters
Drosophila constitutive expression promoters
Drosophila tissue-specific promoters
Drosophila inducible expression promoters
Drosophila Pol III promoters
Plant Pol III promoters
C. elegans constitutive expression promoters
C. elegans tissue-specific expression promoters
C. elegans inducible expression promoters
Mini promoter
Fluorescent markers
Chemical light reporter markers
Chromogenic reporter markers
PH-sensitive fluorescent reporter markers
Calcium indicator markers
Yeast screening markers
Antibiotics resistance gene
Read-through linker
Non-read-through linker
Dual Reporter gene
Epitope Tags
Purification Tags
Protein cleavage sites
Signal peptides
Degron Tags
Dimer domain
Cell penetrating peptide
BE3
Cas12a
Cas9
dCas9
Chimeric antigen receptor domain
Antibody heavy chain constant region
Antibody light chain constant region
Transcriptional regulatory protein
Tet regulatory protein
DNA Recombinase
Drug DNA recombinase
Regulatory elements
Enhancer elements
Standard elements Library
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Promoter
All
C. elegans constitutive expression promoters
C. elegans inducible expression promoters
Drosophila constitutive expression promoters
Drosophila inducible expression promoters
Drosophila Pol III promoters
Mammalian constitutive expression promoters
Mammalian inducible expression promoters
Mini promoter
Plant constitutive expression promoters
Plant Pol III promoters
Yeast constitutive expression promoters
Yeast inducible expression promoters
Zebrafish constitutive expression promoters
Zebrafish inducible expression promoters
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Sequence
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All
C. elegans tissue-specific expression promoters
Drosophila tissue-specific promoters
Mammalian tissue-specific expression promoters
All Tissue Specificity
Adult smooth muscle
Blood
Blood vessels
Bone
Bone and cartilage
Bone marrow
Bone, tooth and tendon
Brain
Brain, hippocampus, cerebellum, retina
Cartilage
Embryonic developing pancreas and adult pancreatic islets
Embryonic heart
Embryonic inner cell mass
Epidermis
Fat
Forebrain
Heart
Kidney
Liver
Lung
Lung, heart, ovary, spleen and kidney glomeruli
Mammary gland
Mouse skeletal and heart muscles
Mouse skeletal, heart, and smooth muscle
Muscle
Nervous system
Nervous system at embryonic stage
Nervous systems at embryonic and early postnatal stages
Nervous systems, testis and thymus
Ocelli
Pancreas
Retina
Spinal cord
Tumor
All Cell Type Specificity
Adipocytes
Alveolar epithelial cells of mammary tissue
Astrocytes
AT II cells (alveolar type II epithelial cells) and Clara cells (bronchiolar epithelial cells)
Body muscle cells
CA1 pyramidal cells of the hippocampus
Cardiomyocytes
Chondrocytes
Cone photoreceptor
Cone photoreceptor in non-human primate
Developing neurons but not astrocytes or oligodendrocytes
Differentiated postmitotic striated muscle cells
Dopaminergic neurons
Ductal cells of the salivary gland, mammary epithelial cells
Early osteoblasts
Endothelial cells
Hepatocytes
Immortalized and cancer cells
Keratinocytes
Mature hepatocytes
Mature myeloid cell lines (neutrophils, monocytes and macrophages)
Mature neurons
Melanocytes and melanoma cells
Microglia
Monocytes and macrophages
Motor neurons
Myoblasts
Myocytes
Neural stem/progenitor cells
Neurons
Ocellar photoreceptor cells
Oligodendrocytes and Schwann cells
ON-bipolar cells
Osteoblasts and fibroblasts
Osteoblasts, osteocytes and hypertrophic chondrocytes
Pancreatic progenitor cells and pancreatic β cells
Pancreatic β cells
Pan-neuronal
Pharyngeal muscle cells
Pluripotent stem cells such as embryonic stem cells
Podocytes
Pyramidal neurons
Rod photoreceptor
RPE
Sperm
Various CNS neurons
Various neurons
Vascular endothelial cells
Vascular smooth muscle cells
β cells
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Tissue Specificity
Cell Type Specificity
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Sequence
Description
Tissue Specificity
Cell Type Specificity
References
Type
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Marker genes
Fluorescent markers
All Structure
Dimer
Monomer
Monomer (may form weak dimer)
Monomer (nonaggregating tandem dimer)
Tetramer
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Color
Structure
Maximum Excitation(nm)
Maximum Emission(nm)
Brightness(% of EGFP)
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Name
Sequence
Description
Application Notes
Color
Structure
Maximum Excitation(nm)
Maximum Emission(nm)
Brightness(% of EGFP)
References
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Chemical light reporter markers
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Sequence
Description
Max Emission
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Name
Sequence
Description
Max Emission
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Chromogenic reporter markers
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Sequence
Description
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Substrate
Color
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Description
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Substrate
Color
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PH-sensitive fluorescent reporter markers
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Description
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Name
Sequence
Description
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Calcium indicator markers
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Name Descending
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Sequence
Description
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Name
Sequence
Description
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Yeast screening markers
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Sequence
Description
Auxotrophic Complementation
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Name
Sequence
Description
Auxotrophic Complementation
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Antibiotics resistance gene
All Selection Drug
Blasticidin
Bleomycin, phleomycin or Zeocin(TM)
G418 or Kanamycin
Geneticin (G418)
Glufosinate (phosphinothricin)
Hygromycin B
Puromycin
All Usage
Positive selection
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Description
Selection Drug
Usage
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Sequence
Description
Selection Drug
Usage
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ORF linker
All
All
Fusion protein linker
2A peptide
Non-read-through linker
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Description
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Name
Sequence
Description
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Dual Reporter gene
All Selection Drug
Blasticidin
Bleomycin, phleomycin or Zeocin(TM)
Geneticin (G418)
Hygromycin B
Puromycin
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Sequence
Description
荧光
Selection Drug
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Name
Sequence
Description
荧光
Selection Drug
Application Notes
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Protein Tag
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Cell penetrating peptide
Degron Tags
Dimer domain
Epitope Tags
Protein cleavage sites
Purification Tags
Signal peptides
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Cas gene
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BE3
Cas12a
Cas9
dCas9
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Sequence
Description
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PAM Sequence
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Type
Name
Sequence
Description
Application Notes
PAM Sequence
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Type
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Antibody associated element
All
Antibody heavy chain constant region
Antibody light chain constant region
Chimeric antigen receptor domain
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Description
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Name
Sequence
Description
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Expression regulatory gene
All
DNA Recombinase
Drug DNA recombinase
Tet regulatory protein
Transcriptional regulatory protein
All Target Sites
CuO
FRT and its variants (e.g. FRT3, FRT5)
Inverted/direct repeats (IR/DR) of Mos1 transposon
Inverted/direct repeats (IR/DR) of Sleeping Beauty transposon
LacO
LoxP and its variants (e.g. LoxN, Lox2272, Lox511, Lox66, Lox71)
PiggyBac inverted terminal repeat (ITR)
Rox and its variants (e.g. Rox4R, Rox6R, Rox2N )
SunTag
Tol2 invertet terminal repeat (ITR)
TRE
TRE3G
UAS
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Target Sites
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Name
Sequence
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Target Sites
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Regulatory elements
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Sequence
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Enhancer elements
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Sequence
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Standard elements Library
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5'UTR
CDS
enhancer
exon
gene
intron
LTR
misc_feature
misc_recomb
misc_RNA
misc_signal
mobile_element
oriT
polyA_signal
polyA_site
primer_bind
promoter
protein_bind
rep_origin
repeat_region
terminator
tRNA
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CDS
exon
gap
gene
intron
misc_feature
mRNA
primer_bind
promoter
protein_bind
rep_origin
source
terminator
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