ori(188-776) ori ori(188-776) ori ori(188-776) AmpR(947-1807) AmpR AmpR(947-1807) AmpR AmpR(947-1807) AmpR promoter(1808-1912) AmpR promoter AmpR promoter(1808-1912) AmpR promoter AmpR promoter(1808-1912) AmpR promoter AmpR promoter(1808-1912) f1 ori(1938-2393) f1 ori f1 ori(1938-2393) f1 ori f1 ori(1938-2393) M13 fwd(2535-2551) M13 fwd M13 fwd(2535-2551) M13 fwd M13 fwd(2535-2551) M13 fwd M13 fwd(2535-2551) T7 promoter(2561-2579) T7 promoter T7 promoter(2561-2579) T7 promoter T7 promoter(2561-2579) T7 promoter T7 promoter(2561-2579) SV40 ori(2600-2735) SV40 ori SV40 ori(2600-2735) SV40 ori SV40 ori(2600-2735) SV40 ori SV40 ori(2600-2735) SV40 poly(A) signal(2775-2896) SV40 poly(A) signal SV40 poly(A) signal(2775-2896) SV40 poly(A) signal SV40 poly(A) signal(2775-2896) SV40 poly(A) signal SV40 poly(A) signal(2775-2896) 3' LTR (ΔU3)(2968-3201) 3' LTR (ΔU3) 3' LTR (ΔU3)(2968-3201) 3' LTR (ΔU3) 3' LTR (ΔU3)(2968-3201) 3' LTR (ΔU3) 3' LTR (ΔU3)(2968-3201) PuroR(3329-3928) PuroR PuroR(3329-3928) PuroR PuroR(3329-3928) hPGK promoter(3950-4460) hPGK promoter hPGK promoter(3950-4460) hPGK promoter hPGK promoter(3950-4460) hPGK promoter hPGK promoter(3950-4460) cPPT/CTS(4509-4626) cPPT/CTS cPPT/CTS(4509-4626) cPPT/CTS cPPT/CTS(4509-4626) cPPT/CTS cPPT/CTS(4509-4626) U6 promoter(6540-6788) U6 promoter U6 promoter(6540-6788) U6 promoter U6 promoter(6540-6788) U6 promoter U6 promoter(6540-6788) gp41 peptide(7086-7130) gp41 peptide gp41 peptide(7086-7130) gp41 peptide gp41 peptide(7086-7130) gp41 peptide gp41 peptide(7086-7130) RRE(7315-7548) RRE RRE(7315-7548) RRE RRE(7315-7548) HIV-1 Ψ(8041-8166) HIV-1 Ψ HIV-1 Ψ(8041-8166) HIV-1 Ψ HIV-1 Ψ(8041-8166) HIV-1 Ψ HIV-1 Ψ(8041-8166) 5' LTR (truncated)(8213-8393) 5' LTR (truncated) 5' LTR (truncated)(8213-8393) 5' LTR (truncated) 5' LTR (truncated)(8213-8393) ...5' LTR 5' LTR (truncated)(8213-8393) RSV promoter(8394-8620) RSV promoter RSV promoter(8394-8620) RSV promoter RSV promoter(8394-8620) ...RSV pro RSV promoter(8394-8620) T3 promoter(8648-8666) T3 promoter T3 promoter(8648-8666) T3 promoter T3 promoter(8648-8666) T3 promoter T3 promoter(8648-8666) M13 rev(8687-8703) M13 rev M13 rev(8687-8703) M13 rev M13 rev(8687-8703) M13 rev M13 rev(8687-8703) lac operator(8711-8727) lac operator lac operator(8711-8727) lac operator lac operator(8711-8727) lac operator lac operator(8711-8727) lac promoter(8735-8765) lac promoter lac promoter(8735-8765) lac promoter lac promoter(8735-8765) lac promoter lac promoter(8735-8765) CAP binding site(8780-8801) CAP binding site CAP binding site(8780-8801) CAP binding site CAP binding site(8780-8801) CAP bin... CAP binding site(8780-8801) M13 rev(8689-8705) M13 rev M13 rev(8689-8705) M13 rev M13 rev(8689-8705) M13 rev M13 rev(8689-8705) M13 fwd(2537-2553) M13 fwd M13 fwd(2537-2553) M13 fwd M13 fwd(2537-2553) M13 fwd M13 fwd(2537-2553) lac operator(8713-8729) lac operator lac operator(8713-8729) lac operator lac operator(8713-8729) lac operator lac operator(8713-8729) T7 promoter(2563-2581) T7 promoter T7 promoter(2563-2581) T7 promoter T7 promoter(2563-2581) T7 promoter T7 promoter(2563-2581) T3 promoter(8650-8668) T3 promoter T3 promoter(8650-8668) T3 promoter T3 promoter(8650-8668) T3 promoter T3 promoter(8650-8668) CAP binding site(8782-8803) CAP binding site CAP binding site(8782-8803) CAP binding site CAP binding site(8782-8803) CAP bin... CAP binding site(8782-8803) lac promoter(8737-8767) lac promoter lac promoter(8737-8767) lac promoter lac promoter(8737-8767) lac pro... lac promoter(8737-8767) AmpR promoter(1810-1914) AmpR promoter AmpR promoter(1810-1914) AmpR promoter AmpR promoter(1810-1914) AmpR promoter AmpR promoter(1810-1914) cPPT/CTS(4511-4628) cPPT/CTS cPPT/CTS(4511-4628) cPPT/CTS cPPT/CTS(4511-4628) cPPT/CTS cPPT/CTS(4511-4628) HIV-1 Ψ(8043-8168) HIV-1 Ψ HIV-1 Ψ(8043-8168) HIV-1 Ψ HIV-1 Ψ(8043-8168) HIV-1 Ψ HIV-1 Ψ(8043-8168) SV40 ori(2602-2737) SV40 ori SV40 ori(2602-2737) SV40 ori SV40 ori(2602-2737) SV40 ori SV40 ori(2602-2737) RSV promoter(8396-8624) RSV promoter RSV promoter(8396-8624) RSV promoter RSV promoter(8396-8624) ...RSV pro RSV promoter(8396-8624) RRE(7317-7550) RRE RRE(7317-7550) RRE RRE(7317-7550) 3' LTR (ΔU3)(2970-3203) 3' LTR (ΔU3) 3' LTR (ΔU3)(2970-3203) 3' LTR (ΔU3) 3' LTR (ΔU3)(2970-3203) 3' LTR (ΔU3) 3' LTR (ΔU3)(2970-3203) hPGK promoter(3952-4462) hPGK promoter hPGK promoter(3952-4462) hPGK promoter hPGK promoter(3952-4462) hPGK promoter hPGK promoter(3952-4462)