3' LTR(1-634) 3' LTR 3' LTR(1-634) 3' LTR 3' LTR(1-634) HIV-1 ψ(681-806) HIV-1 ψ HIV-1 ψ(681-806) HIV-1 ψ HIV-1 ψ(681-806) HIV-1 ψ HIV-1 ψ(681-806) HIV-1 RRE(1303-1536) HIV-1 RRE HIV-1 RRE(1303-1536) HIV-1 RRE HIV-1 RRE(1303-1536) HIV-1 RRE HIV-1 RRE(1303-1536) CMV enhancer(2199-2504) CMV enhancer CMV enhancer(2199-2504) CMV enhancer CMV enhancer(2199-2504) CMV enhancer CMV enhancer(2199-2504) CMV promoter(2505-2708) CMV promoter CMV promoter(2505-2708) CMV promoter CMV promoter(2505-2708) CMV promoter CMV promoter(2505-2708) mCherry(2807-3514) mCherry mCherry(2807-3514) mCherry mCherry(2807-3514) MCS(3515-3580) MCS MCS(3515-3580) MCS MCS(3515-3580) MCS MCS(3515-3580) PGK promoter(3613-4112) PGK promoter PGK promoter(3613-4112) PGK promoter PGK promoter(3613-4112) PGK pro... PGK promoter(3613-4112) PuroR(4133-4732) PuroR PuroR(4133-4732) PuroR PuroR(4133-4732) WPRE(4746-5334) WPRE WPRE(4746-5334) WPRE WPRE(4746-5334) Factor Xa site(5217-5228) Factor Xa site Factor Xa site(5217-5228) Factor Xa site Factor Xa site(5217-5228) Factor Xa site Factor Xa site(5217-5228) 3' LTR(5541-6174) 3' LTR 3' LTR(5541-6174) 3' LTR 3' LTR(5541-6174) M13 rev(6303-6319) M13 rev M13 rev(6303-6319) M13 rev M13 rev(6303-6319) M13 rev M13 rev(6303-6319) lac operator(6327-6343) lac operator lac operator(6327-6343) lac operator lac operator(6327-6343) lac operator lac operator(6327-6343) lac promoter(6351-6381) lac promoter lac promoter(6351-6381) lac promoter lac promoter(6351-6381) lac promoter lac promoter(6351-6381) ori(6705-7293) ori ori(6705-7293) ori ori(6705-7293) AmpR(7464-8324) AmpR AmpR(7464-8324) AmpR AmpR(7464-8324) AmpR promoter(8325-8429) AmpR promoter AmpR promoter(8325-8429) AmpR promoter AmpR promoter(8325-8429) AmpR promoter AmpR promoter(8325-8429) SV40 poly(A) signal(8477-8611) SV40 poly(A) signal SV40 poly(A) signal(8477-8611) SV40 poly(A) signal SV40 poly(A) signal(8477-8611) SV40 poly(A) signal SV40 poly(A) signal(8477-8611) Factor Xa site(5219-5230) Factor Xa site Factor Xa site(5219-5230) Factor Xa site Factor Xa site(5219-5230) Factor Xa site Factor Xa site(5219-5230) M13 rev(6305-6321) M13 rev M13 rev(6305-6321) M13 rev M13 rev(6305-6321) M13 rev M13 rev(6305-6321) lac operator(6329-6345) lac operator lac operator(6329-6345) lac operator lac operator(6329-6345) lac operator lac operator(6329-6345) CAP binding site(6398-6419) CAP binding site CAP binding site(6398-6419) CAP binding site CAP binding site(6398-6419) CAP binding site CAP binding site(6398-6419) lac promoter(6353-6383) lac promoter lac promoter(6353-6383) lac promoter lac promoter(6353-6383) lac promoter lac promoter(6353-6383) MCS(3517-3582) MCS MCS(3517-3582) MCS MCS(3517-3582) MCS MCS(3517-3582) AmpR promoter(8327-8431) AmpR promoter AmpR promoter(8327-8431) AmpR promoter AmpR promoter(8327-8431) AmpR promoter AmpR promoter(8327-8431) HIV-1 Ψ(683-808) HIV-1 Ψ HIV-1 Ψ(683-808) HIV-1 Ψ HIV-1 Ψ(683-808) HIV-1 Ψ HIV-1 Ψ(683-808) SV40 poly(A) signal(8479-8613) SV40 poly(A) signal SV40 poly(A) signal(8479-8613) SV40 poly(A) signal SV40 poly(A) signal(8479-8613) SV40 poly(A) signal SV40 poly(A) signal(8479-8613) CMV promoter(2507-2710) CMV promoter CMV promoter(2507-2710) CMV promoter CMV promoter(2507-2710) CMV promoter CMV promoter(2507-2710) RRE(1305-1538) RRE RRE(1305-1538) RRE RRE(1305-1538) PGK promoter(3615-4114) PGK promoter PGK promoter(3615-4114) PGK promoter PGK promoter(3615-4114) PGK promoter PGK promoter(3615-4114) WPRE(4748-5336) WPRE WPRE(4748-5336) WPRE WPRE(4748-5336)