3' LTR(1-634) 3' LTR 3' LTR(1-634) 3' LTR 3' LTR(1-634) HIV-1 ψ(681-806) HIV-1 ψ HIV-1 ψ(681-806) HIV-1 ψ HIV-1 ψ(681-806) HIV-1 ψ HIV-1 ψ(681-806) HIV-1 RRE(1303-1536) HIV-1 RRE HIV-1 RRE(1303-1536) HIV-1 RRE HIV-1 RRE(1303-1536) HIV-1 RRE HIV-1 RRE(1303-1536) CMV enhancer(2199-2504) CMV enhancer CMV enhancer(2199-2504) CMV enhancer CMV enhancer(2199-2504) CMV enhancer CMV enhancer(2199-2504) CMV promoter(2505-2708) CMV promoter CMV promoter(2505-2708) CMV promoter CMV promoter(2505-2708) CMV promoter CMV promoter(2505-2708) MCS(2793-2873) MCS MCS(2793-2873) MCS MCS(2793-2873) MCS MCS(2793-2873) AcGFP1(2875-3594) AcGFP1 AcGFP1(2875-3594) AcGFP1 AcGFP1(2875-3594) PGK promoter(3622-4121) PGK promoter PGK promoter(3622-4121) PGK promoter PGK promoter(3622-4121) PGK promoter PGK promoter(3622-4121) PuroR(4142-4741) PuroR PuroR(4142-4741) PuroR PuroR(4142-4741) WPRE(4755-5343) WPRE WPRE(4755-5343) WPRE WPRE(4755-5343) Factor Xa site(5226-5237) Factor Xa site Factor Xa site(5226-5237) Factor Xa site Factor Xa site(5226-5237) Factor Xa site Factor Xa site(5226-5237) 3' LTR(5550-6183) 3' LTR 3' LTR(5550-6183) 3' LTR 3' LTR(5550-6183) M13 rev(6312-6328) M13 rev M13 rev(6312-6328) M13 rev M13 rev(6312-6328) M13 rev M13 rev(6312-6328) lac operator(6336-6352) lac operator lac operator(6336-6352) lac operator lac operator(6336-6352) lac operator lac operator(6336-6352) lac promoter(6360-6390) lac promoter lac promoter(6360-6390) lac promoter lac promoter(6360-6390) lac promoter lac promoter(6360-6390) ori(6714-7302) ori ori(6714-7302) ori ori(6714-7302) AmpR(7473-8333) AmpR AmpR(7473-8333) AmpR AmpR(7473-8333) AmpR promoter(8334-8438) AmpR promoter AmpR promoter(8334-8438) AmpR promoter AmpR promoter(8334-8438) AmpR promoter AmpR promoter(8334-8438) SV40 poly(A) signal(8486-8620) SV40 poly(A) signal SV40 poly(A) signal(8486-8620) SV40 poly(A) signal SV40 poly(A) signal(8486-8620) SV40 poly(A) signal SV40 poly(A) signal(8486-8620) Factor Xa site(5228-5239) Factor Xa site Factor Xa site(5228-5239) Factor Xa site Factor Xa site(5228-5239) Factor Xa site Factor Xa site(5228-5239) M13 rev(6314-6330) M13 rev M13 rev(6314-6330) M13 rev M13 rev(6314-6330) M13 rev M13 rev(6314-6330) lac operator(6338-6354) lac operator lac operator(6338-6354) lac operator lac operator(6338-6354) lac operator lac operator(6338-6354) CAP binding site(6407-6428) CAP binding site CAP binding site(6407-6428) CAP binding site CAP binding site(6407-6428) CAP binding site CAP binding site(6407-6428) lac promoter(6362-6392) lac promoter lac promoter(6362-6392) lac promoter lac promoter(6362-6392) lac promoter lac promoter(6362-6392) MCS(2798-2875) MCS MCS(2798-2875) MCS MCS(2798-2875) MCS MCS(2798-2875) MCS(2795-2875) MCS MCS(2795-2875) MCS MCS(2795-2875) MCS MCS(2795-2875) AmpR promoter(8336-8440) AmpR promoter AmpR promoter(8336-8440) AmpR promoter AmpR promoter(8336-8440) AmpR promoter AmpR promoter(8336-8440) HIV-1 Ψ(683-808) HIV-1 Ψ HIV-1 Ψ(683-808) HIV-1 Ψ HIV-1 Ψ(683-808) HIV-1 Ψ HIV-1 Ψ(683-808) SV40 poly(A) signal(8488-8622) SV40 poly(A) signal SV40 poly(A) signal(8488-8622) SV40 poly(A) signal SV40 poly(A) signal(8488-8622) SV40 poly(A) signal SV40 poly(A) signal(8488-8622) CMV promoter(2507-2710) CMV promoter CMV promoter(2507-2710) CMV promoter CMV promoter(2507-2710) CMV promoter CMV promoter(2507-2710) RRE(1305-1538) RRE RRE(1305-1538) RRE RRE(1305-1538) PGK promoter(3624-4123) PGK promoter PGK promoter(3624-4123) PGK promoter PGK promoter(3624-4123) PGK promoter PGK promoter(3624-4123) WPRE(4757-5345) WPRE WPRE(4757-5345) WPRE WPRE(4757-5345) AcGFP1(2877-3596) AcGFP1 AcGFP1(2877-3596) AcGFP1 AcGFP1(2877-3596)