f1 ori(12-467) f1 ori f1 ori(12-467) f1 ori f1 ori(12-467) AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR(599-1459) AmpR AmpR(599-1459) AmpR AmpR(599-1459) ori(1630-2218) ori ori(1630-2218) ori ori(1630-2218) rop(2648-2839) rop rop(2648-2839) rop rop(2648-2839) T7 promoter(3348-3366) T7 promoter T7 promoter(3348-3366) T7 promoter T7 promoter(3348-3366) T7 promoter T7 promoter(3348-3366) T7 tag (gene 10 leader)(3428-3460) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(3428-3460) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(3428-3460) ...T7 tag T7 tag (gene 10 leader)(3428-3460) 6xHis(3509-3526) 6xHis 6xHis(3509-3526) 6xHis 6xHis(3509-3526) 6xHis 6xHis(3509-3526) T7 terminator(3593-3640) T7 terminator T7 terminator(3593-3640) T7 terminator T7 terminator(3593-3640) T7 terminator T7 terminator(3593-3640) T7 promoter(3350-3368) T7 promoter T7 promoter(3350-3368) T7 promoter T7 promoter(3350-3368) ...T7 prom T7 promoter(3350-3368) T7 tag (gene 10 leader)(3430-3462) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(3430-3462) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(3430-3462) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(3430-3462) T7 terminator(3595-3642) T7 terminator T7 terminator(3595-3642) T7 terminator T7 terminator(3595-3642) T7 terminator T7 terminator(3595-3642) AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) rop(2650-2841) rop rop(2650-2841) rop rop(2650-2841) f1 ori(14-469) f1 ori f1 ori(14-469) f1 ori f1 ori(14-469) ori(1632-2220) ori ori(1632-2220) ori ori(1632-2220)