f1 ori(12-467) f1 ori f1 ori(12-467) f1 ori f1 ori(12-467) AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR(599-1459) AmpR AmpR(599-1459) AmpR AmpR(599-1459) ori(1630-2218) ori ori(1630-2218) ori ori(1630-2218) rop(2648-2839) rop rop(2648-2839) rop rop(2648-2839) T7 promoter(3348-3366) T7 promoter T7 promoter(3348-3366) T7 promoter T7 promoter(3348-3366) T7 promoter T7 promoter(3348-3366) pelB signal sequence(3428-3493) pelB signal sequence pelB signal sequence(3428-3493) pelB signal sequence pelB signal sequence(3428-3493) ...pelB si pelB signal sequence(3428-3493) 6xHis(3560-3577) 6xHis 6xHis(3560-3577) 6xHis 6xHis(3560-3577) 6xHis 6xHis(3560-3577) T7 terminator(3644-3691) T7 terminator T7 terminator(3644-3691) T7 terminator T7 terminator(3644-3691) ...T7 term T7 terminator(3644-3691) T7 promoter(3350-3368) T7 promoter T7 promoter(3350-3368) T7 promoter T7 promoter(3350-3368) T7 promoter T7 promoter(3350-3368) T7 terminator(3646-3693) T7 terminator T7 terminator(3646-3693) T7 terminator T7 terminator(3646-3693) ...T7 term T7 terminator(3646-3693) pelB signal sequence(3430-3495) pelB signal sequence pelB signal sequence(3430-3495) pelB signal sequence pelB signal sequence(3430-3495) ...pelB si pelB signal sequence(3430-3495) AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) rop(2650-2841) rop rop(2650-2841) rop rop(2650-2841) f1 ori(14-469) f1 ori f1 ori(14-469) f1 ori f1 ori(14-469) ori(1632-2220) ori ori(1632-2220) ori ori(1632-2220)