lac promoter(142-172) lac promoter lac promoter(142-172) lac promoter lac promoter(142-172) lac pro... lac promoter(142-172) lac operator(180-196) lac operator lac operator(180-196) lac operator lac operator(180-196) lac operator lac operator(180-196) M13 rev(204-220) M13 rev M13 rev(204-220) M13 rev M13 rev(204-220) M13 rev M13 rev(204-220) MCS(233-289) MCS MCS(233-289) MCS MCS(233-289) M13 fwd(290-306) M13 fwd M13 fwd(290-306) M13 fwd M13 fwd(290-306) M13 fwd M13 fwd(290-306) lacZα(216-539) lacZα lacZα(216-539) lacZα lacZα(216-539) AmpR promoter(780-884) AmpR promoter AmpR promoter(780-884) AmpR promoter AmpR promoter(780-884) AmpR promoter AmpR promoter(780-884) AmpR(885-1745) AmpR AmpR(885-1745) AmpR AmpR(885-1745) ori(1916-2504) ori ori(1916-2504) ori ori(1916-2504) M13 rev(206-222) M13 rev M13 rev(206-222) M13 rev M13 rev(206-222) M13 rev M13 rev(206-222) M13 fwd(292-308) M13 fwd M13 fwd(292-308) M13 fwd M13 fwd(292-308) M13 fwd M13 fwd(292-308) lac operator(182-198) lac operator lac operator(182-198) lac operator lac operator(182-198) lac ope... lac operator(182-198) CAP binding site(108-129) CAP binding site CAP binding site(108-129) CAP binding site CAP binding site(108-129) CAP bin... CAP binding site(108-129) lac promoter(144-174) lac promoter lac promoter(144-174) lac promoter lac promoter(144-174) lac pro... lac promoter(144-174) MCS(235-291) MCS MCS(235-291) MCS MCS(235-291) MCS MCS(235-291) MCS(235-291) MCS MCS(235-291) MCS MCS(235-291) MCS MCS(235-291) AmpR promoter(782-886) AmpR promoter AmpR promoter(782-886) AmpR promoter AmpR promoter(782-886) AmpR promoter AmpR promoter(782-886) lacZα(218-541) lacZα lacZα(218-541) lacZα lacZα(218-541)