lac operator(3-27) lac operator lac operator(3-27) lac operator lac operator(3-27) lac ope... lac operator(3-27) 8xHis(80-103) 8xHis 8xHis(80-103) 8xHis 8xHis(80-103) 8xHis 8xHis(80-103) MCS1(149-199) MCS1 MCS1(149-199) MCS1 MCS1(149-199) MCS1 MCS1(149-199) T7 promoter(245-263) T7 promoter T7 promoter(245-263) T7 promoter T7 promoter(245-263) T7 promoter T7 promoter(245-263) lac operator(264-288) lac operator lac operator(264-288) lac operator lac operator(264-288) lac ope... lac operator(264-288) MCS2(328-390) MCS2 MCS2(328-390) MCS2 MCS2(328-390) MCS2 MCS2(328-390) S-Tag(397-441) S-Tag S-Tag(397-441) S-Tag S-Tag(397-441) S-Tag S-Tag(397-441) T7 terminator(493-540) T7 terminator T7 terminator(493-540) T7 terminator T7 terminator(493-540) T7 terminator T7 terminator(493-540) CmR(760-1419) CmR CmR(760-1419) CmR CmR(760-1419) cat promoter(1420-1522) cat promoter cat promoter(1420-1522) cat promoter cat promoter(1420-1522) cat promoter cat promoter(1420-1522) T7 promoter(4023-2) T7 promoter T7 promoter(4023-2) T7 promoter T7 promoter(4023-2) T7 promoter T7 promoter(4023-2) p15A ori(2048-2592) p15A ori p15A ori(2048-2592) p15A ori p15A ori(2048-2592) lacI(2817-3899) lacI lacI(2817-3899) lacI lacI(2817-3899) lacI promoter(3900-3977) lacI promoter lacI promoter(3900-3977) lacI promoter lacI promoter(3900-3977) lacI promoter lacI promoter(3900-3977) lac operator(9-25) lac operator lac operator(9-25) lac operator lac operator(9-25) lac ope... lac operator(9-25) lac operator(270-286) lac operator lac operator(270-286) lac operator lac operator(270-286) lac ope... lac operator(270-286) T7 promoter(247-265) T7 promoter T7 promoter(247-265) T7 promoter T7 promoter(247-265) T7 prom... T7 promoter(247-265) lac operator(5-29) lac operator lac operator(5-29) lac operator lac operator(5-29) lac ope... lac operator(5-29) lac operator(266-290) lac operator lac operator(266-290) lac operator lac operator(266-290) lac ope... lac operator(266-290) T7 terminator(495-542) T7 terminator T7 terminator(495-542) T7 terminator T7 terminator(495-542) T7 terminator T7 terminator(495-542) lacI promoter(3902-3979) lacI promoter lacI promoter(3902-3979) lacI promoter lacI promoter(3902-3979) lacI promoter lacI promoter(3902-3979) cat promoter(1422-1524) cat promoter cat promoter(1422-1524) cat promoter cat promoter(1422-1524) cat promoter cat promoter(1422-1524)