AmpR promoter(104-208) AmpR promoter AmpR promoter(104-208) AmpR promoter AmpR promoter(104-208) AmpR promoter AmpR promoter(104-208) AmpR(209-1069) AmpR AmpR(209-1069) AmpR AmpR(209-1069) ori(1240-1828) ori ori(1240-1828) ori ori(1240-1828) rop(2258-2449) rop rop(2258-2449) rop rop(2258-2449) lacI(3761-4843) lacI lacI(3761-4843) lacI lacI(3761-4843) lacI promoter(4844-4921) lacI promoter lacI promoter(4844-4921) lacI promoter lacI promoter(4844-4921) lacI promoter lacI promoter(4844-4921) T7 promoter(5230-5248) T7 promoter T7 promoter(5230-5248) T7 promoter T7 promoter(5230-5248) T7 promoter T7 promoter(5230-5248) lac operator(5249-5273) lac operator lac operator(5249-5273) lac operator lac operator(5249-5273) ...lac ope lac operator(5249-5273) 6xHis(5329-5346) 6xHis 6xHis(5329-5346) 6xHis 6xHis(5329-5346) 6xHis 6xHis(5329-5346) thrombin site(5356-5373) thrombin site thrombin site(5356-5373) thrombin site thrombin site(5356-5373) ...thrombi thrombin site(5356-5373) T7 terminator(5449-5496) T7 terminator T7 terminator(5449-5496) T7 terminator T7 terminator(5449-5496) ...T7 term T7 terminator(5449-5496) tet promoter(5671-5699) tet promoter tet promoter(5671-5699) tet promoter tet promoter(5671-5699) ...tet pro tet promoter(5671-5699) lac operator(5255-5271) lac operator lac operator(5255-5271) lac operator lac operator(5255-5271) ...lac ope lac operator(5255-5271) thrombin site(5358-5375) thrombin site thrombin site(5358-5375) thrombin site thrombin site(5358-5375) ...thrombi thrombin site(5358-5375) T7 promoter(5232-5250) T7 promoter T7 promoter(5232-5250) T7 promoter T7 promoter(5232-5250) ...T7 prom T7 promoter(5232-5250) lac operator(5251-5275) lac operator lac operator(5251-5275) lac operator lac operator(5251-5275) ...lac ope lac operator(5251-5275) tet promoter(5673-5701) tet promoter tet promoter(5673-5701) tet promoter tet promoter(5673-5701) tet promoter tet promoter(5673-5701) T7 terminator(5451-5498) T7 terminator T7 terminator(5451-5498) T7 terminator T7 terminator(5451-5498) ...T7 term T7 terminator(5451-5498) lacI promoter(4846-4923) lacI promoter lacI promoter(4846-4923) lacI promoter lacI promoter(4846-4923) lacI promoter lacI promoter(4846-4923) AmpR promoter(106-210) AmpR promoter AmpR promoter(106-210) AmpR promoter AmpR promoter(106-210) AmpR promoter AmpR promoter(106-210) rop(2260-2451) rop rop(2260-2451) rop rop(2260-2451) ori(1242-1830) ori ori(1242-1830) ori ori(1242-1830) AmpR(211-1071) AmpR AmpR(211-1071) AmpR AmpR(211-1071)