f1 ori(12-467) f1 ori f1 ori(12-467) f1 ori f1 ori(12-467) AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR(599-1459) AmpR AmpR(599-1459) AmpR AmpR(599-1459) ori(1630-2218) ori ori(1630-2218) ori ori(1630-2218) rop(2648-2839) rop rop(2648-2839) rop rop(2648-2839) lacI(3648-4730) lacI lacI(3648-4730) lacI lacI(3648-4730) lacI promoter(4731-4808) lacI promoter lacI promoter(4731-4808) lacI promoter lacI promoter(4731-4808) lacI promoter lacI promoter(4731-4808) T7 promoter(5117-5135) T7 promoter T7 promoter(5117-5135) T7 promoter T7 promoter(5117-5135) T7 promoter T7 promoter(5117-5135) lac operator(5136-5160) lac operator lac operator(5136-5160) lac operator lac operator(5136-5160) ...lac ope lac operator(5136-5160) pelB signal sequence(5205-5270) pelB signal sequence pelB signal sequence(5205-5270) pelB signal sequence pelB signal sequence(5205-5270) pelB signal sequence pelB signal sequence(5205-5270) 6xHis(5337-5354) 6xHis 6xHis(5337-5354) 6xHis 6xHis(5337-5354) 6xHis 6xHis(5337-5354) T7 terminator(5421-5468) T7 terminator T7 terminator(5421-5468) T7 terminator T7 terminator(5421-5468) T7 terminator T7 terminator(5421-5468) lac operator(5142-5158) lac operator lac operator(5142-5158) lac operator lac operator(5142-5158) ...lac ope lac operator(5142-5158) T7 promoter(5119-5137) T7 promoter T7 promoter(5119-5137) T7 promoter T7 promoter(5119-5137) ...T7 prom T7 promoter(5119-5137) lac operator(5138-5162) lac operator lac operator(5138-5162) lac operator lac operator(5138-5162) ...lac ope lac operator(5138-5162) T7 terminator(5423-5470) T7 terminator T7 terminator(5423-5470) T7 terminator T7 terminator(5423-5470) T7 terminator T7 terminator(5423-5470) pelB signal sequence(5207-5272) pelB signal sequence pelB signal sequence(5207-5272) pelB signal sequence pelB signal sequence(5207-5272) pelB signal sequence pelB signal sequence(5207-5272) lacI promoter(4733-4810) lacI promoter lacI promoter(4733-4810) lacI promoter lacI promoter(4733-4810) lacI promoter lacI promoter(4733-4810) AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) rop(2650-2841) rop rop(2650-2841) rop rop(2650-2841) f1 ori(14-469) f1 ori f1 ori(14-469) f1 ori f1 ori(14-469) ori(1632-2220) ori ori(1632-2220) ori ori(1632-2220)