f1 ori(12-467) f1 ori f1 ori(12-467) f1 ori f1 ori(12-467) KanR(560-1375) KanR KanR(560-1375) KanR KanR(560-1375) ori(1497-2085) ori ori(1497-2085) ori ori(1497-2085) rop(2515-2706) rop rop(2515-2706) rop rop(2515-2706) lacI(3515-4597) lacI lacI(3515-4597) lacI lacI(3515-4597) lacI promoter(4598-4675) lacI promoter lacI promoter(4598-4675) lacI promoter lacI promoter(4598-4675) lacI promoter lacI promoter(4598-4675) T7 promoter(4984-5002) T7 promoter T7 promoter(4984-5002) T7 promoter T7 promoter(4984-5002) T7 promoter T7 promoter(4984-5002) lac operator(5003-5027) lac operator lac operator(5003-5027) lac operator lac operator(5003-5027) lac operator lac operator(5003-5027) T7 tag (gene 10 leader)(5072-5104) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(5072-5104) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(5072-5104) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(5072-5104) 6xHis(5154-5171) 6xHis 6xHis(5154-5171) 6xHis 6xHis(5154-5171) 6xHis 6xHis(5154-5171) T7 terminator(5238-5285) T7 terminator T7 terminator(5238-5285) T7 terminator T7 terminator(5238-5285) T7 terminator T7 terminator(5238-5285) lac operator(5009-5025) lac operator lac operator(5009-5025) lac operator lac operator(5009-5025) lac operator lac operator(5009-5025) T7 promoter(4986-5004) T7 promoter T7 promoter(4986-5004) T7 promoter T7 promoter(4986-5004) T7 promoter T7 promoter(4986-5004) lac operator(5005-5029) lac operator lac operator(5005-5029) lac operator lac operator(5005-5029) lac operator lac operator(5005-5029) T7 tag (gene 10 leader)(5074-5106) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(5074-5106) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(5074-5106) T7 tag (gene 10 leader) T7 tag (gene 10 leader)(5074-5106) T7 terminator(5240-5287) T7 terminator T7 terminator(5240-5287) T7 terminator T7 terminator(5240-5287) T7 term... T7 terminator(5240-5287) lacI promoter(4600-4677) lacI promoter lacI promoter(4600-4677) lacI promoter lacI promoter(4600-4677) lacI promoter lacI promoter(4600-4677) rop(2517-2708) rop rop(2517-2708) rop rop(2517-2708) f1 ori(14-469) f1 ori f1 ori(14-469) f1 ori f1 ori(14-469) ori(1499-2087) ori ori(1499-2087) ori ori(1499-2087)