MCS(5-61) MCS MCS(5-61) MCS MCS(5-61) MCS MCS(5-61) SP6 promoter(68-86) SP6 promoter SP6 promoter(68-86) SP6 promoter SP6 promoter(68-86) SP6 promoter SP6 promoter(68-86) M13 rev(104-120) M13 rev M13 rev(104-120) M13 rev M13 rev(104-120) M13 rev M13 rev(104-120) lac operator(128-144) lac operator lac operator(128-144) lac operator lac operator(128-144) lac operator lac operator(128-144) lac promoter(152-182) lac promoter lac promoter(152-182) lac promoter lac promoter(152-182) lac promoter lac promoter(152-182) ori(506-1094) ori ori(506-1094) ori ori(506-1094) T7 promoter(3181-2) T7 promoter T7 promoter(3181-2) T7 promoter T7 promoter(3181-2) T7 promoter T7 promoter(3181-2) AmpR(1265-2125) AmpR AmpR(1265-2125) AmpR AmpR(1265-2125) AmpR promoter(2126-2230) AmpR promoter AmpR promoter(2126-2230) AmpR promoter AmpR promoter(2126-2230) AmpR promoter AmpR promoter(2126-2230) f1 ori(2562-3017) f1 ori f1 ori(2562-3017) f1 ori f1 ori(2562-3017) M13 fwd(3158-3174) M13 fwd M13 fwd(3158-3174) M13 fwd M13 fwd(3158-3174) M13 fwd M13 fwd(3158-3174) M13 rev(106-122) M13 rev M13 rev(106-122) M13 rev M13 rev(106-122) M13 rev M13 rev(106-122) M13 fwd(3160-3176) M13 fwd M13 fwd(3160-3176) M13 fwd M13 fwd(3160-3176) M13 fwd M13 fwd(3160-3176) lac operator(130-146) lac operator lac operator(130-146) lac operator lac operator(130-146) lac operator lac operator(130-146) SP6 promoter(70-88) SP6 promoter SP6 promoter(70-88) SP6 promoter SP6 promoter(70-88) SP6 promoter SP6 promoter(70-88) CAP binding site(199-220) CAP binding site CAP binding site(199-220) CAP binding site CAP binding site(199-220) CAP binding site CAP binding site(199-220) lac promoter(154-184) lac promoter lac promoter(154-184) lac promoter lac promoter(154-184) lac promoter lac promoter(154-184) MCS(7-63) MCS MCS(7-63) MCS MCS(7-63) MCS MCS(7-63) MCS(7-63) MCS MCS(7-63) MCS MCS(7-63) MCS MCS(7-63) AmpR promoter(2128-2232) AmpR promoter AmpR promoter(2128-2232) AmpR promoter AmpR promoter(2128-2232) AmpR promoter AmpR promoter(2128-2232)