f1 ori(12-467) f1 ori f1 ori(12-467) f1 ori f1 ori(12-467) KanR(560-1375) KanR KanR(560-1375) KanR KanR(560-1375) ori(1497-2085) ori ori(1497-2085) ori ori(1497-2085) rop(2515-2706) rop rop(2515-2706) rop rop(2515-2706) lacI(3515-4597) lacI lacI(3515-4597) lacI lacI(3515-4597) lacI promoter(4598-4675) lacI promoter lacI promoter(4598-4675) lacI promoter lacI promoter(4598-4675) lacI promoter lacI promoter(4598-4675) T7 promoter(4988-5006) T7 promoter T7 promoter(4988-5006) T7 promoter T7 promoter(4988-5006) T7 promoter T7 promoter(4988-5006) lac operator(5007-5031) lac operator lac operator(5007-5031) lac operator lac operator(5007-5031) ...lac ope lac operator(5007-5031) 6xHis(5079-5096) 6xHis 6xHis(5079-5096) 6xHis 6xHis(5079-5096) 6xHis 6xHis(5079-5096) thrombin site(5106-5123) thrombin site thrombin site(5106-5123) thrombin site thrombin site(5106-5123) ...thrombi thrombin site(5106-5123) S-Tag(5130-5174) S-Tag S-Tag(5130-5174) S-Tag S-Tag(5130-5174) S-Tag S-Tag(5130-5174) enterokinase site(5190-5204) enterokinase site enterokinase site(5190-5204) enterokinase site enterokinase site(5190-5204) enterokinase site enterokinase site(5190-5204) 6xHis(5266-5283) 6xHis 6xHis(5266-5283) 6xHis 6xHis(5266-5283) 6xHis 6xHis(5266-5283) T7 terminator(5350-5397) T7 terminator T7 terminator(5350-5397) T7 terminator T7 terminator(5350-5397) T7 terminator T7 terminator(5350-5397) lac operator(5013-5029) lac operator lac operator(5013-5029) lac operator lac operator(5013-5029) lac operator lac operator(5013-5029) T7 promoter(4990-5008) T7 promoter T7 promoter(4990-5008) T7 promoter T7 promoter(4990-5008) T7 promoter T7 promoter(4990-5008) lac operator(5009-5033) lac operator lac operator(5009-5033) lac operator lac operator(5009-5033) ...lac ope lac operator(5009-5033) S-Tag(5132-5176) S-Tag S-Tag(5132-5176) S-Tag S-Tag(5132-5176) S-Tag S-Tag(5132-5176) T7 terminator(5352-5399) T7 terminator T7 terminator(5352-5399) T7 terminator T7 terminator(5352-5399) T7 terminator T7 terminator(5352-5399) lacI promoter(4600-4677) lacI promoter lacI promoter(4600-4677) lacI promoter lacI promoter(4600-4677) lacI promoter lacI promoter(4600-4677) rop(2517-2708) rop rop(2517-2708) rop rop(2517-2708) f1 ori(14-469) f1 ori f1 ori(14-469) f1 ori f1 ori(14-469) ori(1499-2087) ori ori(1499-2087) ori ori(1499-2087)