f1 ori(12-467) f1 ori f1 ori(12-467) f1 ori f1 ori(12-467) AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR promoter AmpR promoter(494-598) AmpR(599-1459) AmpR AmpR(599-1459) AmpR AmpR(599-1459) ori(1630-2218) ori ori(1630-2218) ori ori(1630-2218) rop(2648-2839) rop rop(2648-2839) rop rop(2648-2839) lacI(3648-4730) lacI lacI(3648-4730) lacI lacI(3648-4730) lacI promoter(4731-4808) lacI promoter lacI promoter(4731-4808) lacI promoter lacI promoter(4731-4808) lacI promoter lacI promoter(4731-4808) T7 promoter(5121-5139) T7 promoter T7 promoter(5121-5139) T7 promoter T7 promoter(5121-5139) T7 promoter T7 promoter(5121-5139) lac operator(5140-5164) lac operator lac operator(5140-5164) lac operator lac operator(5140-5164) lac operator lac operator(5140-5164) TrxA(5209-5535) TrxA TrxA(5209-5535) TrxA TrxA(5209-5535) 6xHis(5557-5574) 6xHis 6xHis(5557-5574) 6xHis 6xHis(5557-5574) 6xHis 6xHis(5557-5574) thrombin site(5584-5601) thrombin site thrombin site(5584-5601) thrombin site thrombin site(5584-5601) thrombin site thrombin site(5584-5601) S-Tag(5608-5652) S-Tag S-Tag(5608-5652) S-Tag S-Tag(5608-5652) S-Tag S-Tag(5608-5652) enterokinase site(5668-5682) enterokinase site enterokinase site(5668-5682) enterokinase site enterokinase site(5668-5682) enterokinase site enterokinase site(5668-5682) 6xHis(5744-5761) 6xHis 6xHis(5744-5761) 6xHis 6xHis(5744-5761) 6xHis 6xHis(5744-5761) T7 terminator(5828-5875) T7 terminator T7 terminator(5828-5875) T7 terminator T7 terminator(5828-5875) T7 terminator T7 terminator(5828-5875) lac operator(5146-5162) lac operator lac operator(5146-5162) lac operator lac operator(5146-5162) lac operator lac operator(5146-5162) T7 promoter(5123-5141) T7 promoter T7 promoter(5123-5141) T7 promoter T7 promoter(5123-5141) T7 promoter T7 promoter(5123-5141) lac operator(5142-5166) lac operator lac operator(5142-5166) lac operator lac operator(5142-5166) lac operator lac operator(5142-5166) S-Tag(5610-5654) S-Tag S-Tag(5610-5654) S-Tag S-Tag(5610-5654) S-Tag S-Tag(5610-5654) T7 terminator(5830-5877) T7 terminator T7 terminator(5830-5877) T7 terminator T7 terminator(5830-5877) T7 terminator T7 terminator(5830-5877) lacI promoter(4733-4810) lacI promoter lacI promoter(4733-4810) lacI promoter lacI promoter(4733-4810) lacI promoter lacI promoter(4733-4810) AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) AmpR promoter AmpR promoter(496-600) rop(2650-2841) rop rop(2650-2841) rop rop(2650-2841) TrxA(5211-5537) TrxA TrxA(5211-5537) TrxA TrxA(5211-5537) f1 ori(14-469) f1 ori f1 ori(14-469) f1 ori f1 ori(14-469) ori(1632-2220) ori ori(1632-2220) ori ori(1632-2220)