f1 ori(12-467) f1 ori f1 ori(12-467) f1 ori f1 ori(12-467) KanR(560-1375) KanR KanR(560-1375) KanR KanR(560-1375) ori(1497-2085) ori ori(1497-2085) ori ori(1497-2085) rop(2515-2706) rop rop(2515-2706) rop rop(2515-2706) lacI(3278-4360) lacI lacI(3278-4360) lacI lacI(3278-4360) lacI promoter(4361-4438) lacI promoter lacI promoter(4361-4438) lacI promoter lacI promoter(4361-4438) lacI promoter lacI promoter(4361-4438) T7 promoter(4751-4769) T7 promoter T7 promoter(4751-4769) T7 promoter T7 promoter(4751-4769) T7 promoter T7 promoter(4751-4769) lac operator(4770-4794) lac operator lac operator(4770-4794) lac operator lac operator(4770-4794) lac operator lac operator(4770-4794) GST(4839-5492) GST GST(4839-5492) GST GST(4839-5492) 6xHis(5520-5537) 6xHis 6xHis(5520-5537) 6xHis 6xHis(5520-5537) 6xHis 6xHis(5520-5537) thrombin site(5547-5564) thrombin site thrombin site(5547-5564) thrombin site thrombin site(5547-5564) thrombin site thrombin site(5547-5564) S-Tag(5580-5624) S-Tag S-Tag(5580-5624) S-Tag S-Tag(5580-5624) S-Tag S-Tag(5580-5624) enterokinase site(5655-5669) enterokinase site enterokinase site(5655-5669) enterokinase site enterokinase site(5655-5669) enterokinase site enterokinase site(5655-5669) 8xHis(5761-5784) 8xHis 8xHis(5761-5784) 8xHis 8xHis(5761-5784) 8xHis 8xHis(5761-5784) T7 terminator(5861-5908) T7 terminator T7 terminator(5861-5908) T7 terminator T7 terminator(5861-5908) T7 terminator T7 terminator(5861-5908) lac operator(4776-4792) lac operator lac operator(4776-4792) lac operator lac operator(4776-4792) lac operator lac operator(4776-4792) T7 promoter(4753-4771) T7 promoter T7 promoter(4753-4771) T7 promoter T7 promoter(4753-4771) T7 promoter T7 promoter(4753-4771) lac operator(4772-4796) lac operator lac operator(4772-4796) lac operator lac operator(4772-4796) lac operator lac operator(4772-4796) S-Tag(5582-5626) S-Tag S-Tag(5582-5626) S-Tag S-Tag(5582-5626) S-Tag S-Tag(5582-5626) T7 terminator(5863-5910) T7 terminator T7 terminator(5863-5910) T7 terminator T7 terminator(5863-5910) T7 terminator T7 terminator(5863-5910) lacI promoter(4363-4440) lacI promoter lacI promoter(4363-4440) lacI promoter lacI promoter(4363-4440) lacI promoter lacI promoter(4363-4440) rop(2517-2708) rop rop(2517-2708) rop rop(2517-2708) f1 ori(14-469) f1 ori f1 ori(14-469) f1 ori f1 ori(14-469) ori(1499-2087) ori ori(1499-2087) ori ori(1499-2087)