f1 ori(12-467) f1 ori f1 ori(12-467) f1 ori f1 ori(12-467) AmpR(582-1442) AmpR AmpR(582-1442) AmpR AmpR(582-1442) AmpR promoter(1443-1535) AmpR promoter AmpR promoter(1443-1535) AmpR promoter AmpR promoter(1443-1535) AmpR promoter AmpR promoter(1443-1535) ori(1616-2204) ori ori(1616-2204) ori ori(1616-2204) rop(2634-2825) rop rop(2634-2825) rop rop(2634-2825) lacI(3397-4479) lacI lacI(3397-4479) lacI lacI(3397-4479) lacI promoter(4480-4557) lacI promoter lacI promoter(4480-4557) lacI promoter lacI promoter(4480-4557) lacI promoter lacI promoter(4480-4557) T7 promoter(4870-4888) T7 promoter T7 promoter(4870-4888) T7 promoter T7 promoter(4870-4888) T7 promoter T7 promoter(4870-4888) lac operator(4889-4913) lac operator lac operator(4889-4913) lac operator lac operator(4889-4913) ...lac ope lac operator(4889-4913) 6xHis(4963-4980) 6xHis 6xHis(4963-4980) 6xHis 6xHis(4963-4980) 6xHis 6xHis(4963-4980) enterokinase site(4999-5013) enterokinase site enterokinase site(4999-5013) enterokinase site enterokinase site(4999-5013) ...enterok enterokinase site(4999-5013) S-Tag(5092-5136) S-Tag S-Tag(5092-5136) S-Tag S-Tag(5092-5136) S-Tag S-Tag(5092-5136) T7 terminator(5188-5235) T7 terminator T7 terminator(5188-5235) T7 terminator T7 terminator(5188-5235) ...T7 term T7 terminator(5188-5235) lac operator(4895-4911) lac operator lac operator(4895-4911) lac operator lac operator(4895-4911) ...lac ope lac operator(4895-4911) T7 promoter(4872-4890) T7 promoter T7 promoter(4872-4890) T7 promoter T7 promoter(4872-4890) T7 promoter T7 promoter(4872-4890) lac operator(4891-4915) lac operator lac operator(4891-4915) lac operator lac operator(4891-4915) ...lac ope lac operator(4891-4915) T7 terminator(5190-5237) T7 terminator T7 terminator(5190-5237) T7 terminator T7 terminator(5190-5237) T7 terminator T7 terminator(5190-5237) lacI promoter(4482-4559) lacI promoter lacI promoter(4482-4559) lacI promoter lacI promoter(4482-4559) lacI promoter lacI promoter(4482-4559) rop(2636-2827) rop rop(2636-2827) rop rop(2636-2827) f1 ori(14-469) f1 ori f1 ori(14-469) f1 ori f1 ori(14-469) ori(1618-2206) ori ori(1618-2206) ori ori(1618-2206)