f1 ori(12-467) f1 ori f1 ori(12-467) f1 ori f1 ori(12-467) AmpR(582-1442) AmpR AmpR(582-1442) AmpR AmpR(582-1442) AmpR promoter(1443-1535) AmpR promoter AmpR promoter(1443-1535) AmpR promoter AmpR promoter(1443-1535) AmpR promoter AmpR promoter(1443-1535) ori(1616-2204) ori ori(1616-2204) ori ori(1616-2204) rop(2634-2825) rop rop(2634-2825) rop rop(2634-2825) lacI(3397-4479) lacI lacI(3397-4479) lacI lacI(3397-4479) lacI promoter(4480-4557) lacI promoter lacI promoter(4480-4557) lacI promoter lacI promoter(4480-4557) ...lacI pr lacI promoter(4480-4557) T7 promoter(4870-4888) T7 promoter T7 promoter(4870-4888) T7 promoter T7 promoter(4870-4888) T7 promoter T7 promoter(4870-4888) lac operator(4889-4913) lac operator lac operator(4889-4913) lac operator lac operator(4889-4913) lac operator lac operator(4889-4913) 6xHis(4970-4987) 6xHis 6xHis(4970-4987) 6xHis 6xHis(4970-4987) 6xHis 6xHis(4970-4987) NusA(4994-6478) NusA NusA(4994-6478) NusA NusA(4994-6478) 6xHis(6494-6511) 6xHis 6xHis(6494-6511) 6xHis 6xHis(6494-6511) 6xHis 6xHis(6494-6511) S-Tag(6521-6565) S-Tag S-Tag(6521-6565) S-Tag S-Tag(6521-6565) S-Tag S-Tag(6521-6565) thrombin site(6581-6598) thrombin site thrombin site(6581-6598) thrombin site thrombin site(6581-6598) ...thrombi thrombin site(6581-6598) enterokinase site(6617-6631) enterokinase site enterokinase site(6617-6631) enterokinase site enterokinase site(6617-6631) ...enterok enterokinase site(6617-6631) HSV tag(6743-6775) HSV tag HSV tag(6743-6775) HSV tag HSV tag(6743-6775) HSV tag HSV tag(6743-6775) 6xHis(6782-6799) 6xHis 6xHis(6782-6799) 6xHis 6xHis(6782-6799) 6xHis 6xHis(6782-6799) rrnB T1 terminator(6965-7051) rrnB T1 terminator rrnB T1 terminator(6965-7051) rrnB T1 terminator rrnB T1 terminator(6965-7051) ...rrnB T1 rrnB T1 terminator(6965-7051) T7 terminator(7238-7285) T7 terminator T7 terminator(7238-7285) T7 terminator T7 terminator(7238-7285) ...T7 term T7 terminator(7238-7285) lac operator(4895-4911) lac operator lac operator(4895-4911) lac operator lac operator(4895-4911) ...lac ope lac operator(4895-4911) T7 promoter(4872-4890) T7 promoter T7 promoter(4872-4890) T7 promoter T7 promoter(4872-4890) T7 promoter T7 promoter(4872-4890) lac operator(4891-4915) lac operator lac operator(4891-4915) lac operator lac operator(4891-4915) ...lac ope lac operator(4891-4915) rrnB T2 terminator(7145-7172) rrnB T2 terminator rrnB T2 terminator(7145-7172) rrnB T2 terminator rrnB T2 terminator(7145-7172) ...rrnB T2 rrnB T2 terminator(7145-7172) T7 terminator(7240-7287) T7 terminator T7 terminator(7240-7287) T7 terminator T7 terminator(7240-7287) ...T7 term T7 terminator(7240-7287) lacI promoter(4482-4559) lacI promoter lacI promoter(4482-4559) lacI promoter lacI promoter(4482-4559) ...lacI pr lacI promoter(4482-4559) rrnB T1 terminator(6967-7053) rrnB T1 terminator rrnB T1 terminator(6967-7053) rrnB T1 terminator rrnB T1 terminator(6967-7053) ...rrnB T1 rrnB T1 terminator(6967-7053) rop(2636-2827) rop rop(2636-2827) rop rop(2636-2827) f1 ori(14-469) f1 ori f1 ori(14-469) f1 ori f1 ori(14-469) ori(1618-2206) ori ori(1618-2206) ori ori(1618-2206) NusA(4996-6480) NusA NusA(4996-6480) NusA NusA(4996-6480)